VEGFR-2 蛋白, Cynomolgus (HEK293, His)

VEGFR-2 protein is a tyrosine protein kinase receptor for VEGFA, VEGFC and VEGFD and is critical in angiogenesis, blood vessel development and embryonic hematopoiesis. It promotes endothelial cell function and actin cytoskeletal reorganization. VEGFR-2 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived VEGFR-2 protein, expressed by HEK293 , with C-His labeled tag. The total length of VEGFR-2 Protein, Cynomolgus (HEK293, His) is 745 a.a., with molecular weight of ~115-180 kDa.

VEGFR-2 蛋白是一种酪氨酸蛋白激酶，作为 VEGFA、VEGFC 和 VEGFD 的细胞表面受体，在血管生成、血管发育、血管通透性和胚胎造血的复杂调节中发挥关键作用。它积极促进内皮细胞的增殖、存活、迁移和分化，同时也影响肌动蛋白细胞骨架的重组。某些异构体缺乏跨膜结构域（如异构体 2 和异构体 3），可充当诱饵受体，调节 VEGFA、VEGFC 和/或 VEGFD 信号传导。具体来说，异构体 2 通过限制游离 VEGFA 和/或 VEGFC 的可用性，防止它们与 FLT4 结合，充当 VEGFA 和 VEGFC 介导的淋巴管生成的负调节因子。VEGFR-2 通过异二聚体形成调节 FLT1 和 FLT4 信号传导。血管生长因子与异构体 1 的结合触发多个信号级联反应，包括 PLCG1 的激活，导致二酰甘油和肌醇 1,4,5-三磷酸的产生以及随后蛋白激酶 C 的激活。此外，VEGFR-2 介导MAP激酶信号通路、AKT1信号通路的激活以及PIK3R1的磷酸化，有助于肌动蛋白细胞骨架的重组和PTK2/FAK1的激活。其关键作用包括促进 VEGFA 介导的 NOS2 和 NOS3 诱导，从而导致内皮细胞产生信号分子一氧化氮 (NO)。VEGFR-2 的磷酸化活性包括 PLCG1、FYN、NCK1、NOS3、PIK3R1、PTK2/FAK1 和 SRC，突显其全面参与调节多种细胞过程。

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human VEGF 165 is present at 2 μg/mL can bind Cynomolgus VEGFR-2. The ED₅₀ for this effect is 53.84 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human VEGF 165 is present at 2 μg/mL can bind Cynomolgus VEGFR-2. The ED₅₀ for this effect is 53.84 ng/mL.

Cynomolgus

HEK293

C-His

EHH53800 (A20-E764)

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ASVGLPSVSLDLPRLSIQKDILTIKANTTLQITCRGQRDLDWLWPNNQSGSEQRVEVTECSDGLFCKTLTIPKVIGNDTGAYKCFYRETDLASVIYVYVQDYRSPFIASVSDQHGVVYITENKNKTVVIPCLGSISNLNVSLCARYPEKRFVPDGNRISWDSKKGFTIPSYMISYAGMVFCEAKINDESYQSIMYIVVVVGYRIYDVVLSPSHGVELSVGEKLVLNCTARTELNVGIDFNWEYPSSKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGVTRSDQGLYTCAASSGLMTKKNSTFVRVHEKPFVAFGSGMESLVEATLGERVRIPVKYLGYPPPEIKWYKNGIPLESNHTVKVGHVLTIMEVSERDTGNYTVILTNPISKEKQSHVVSLVVYVPPQIGEKSLISPVDSYQYGTTQTLTCTVYAIPPPHHIHWYWQLEEECPNEPSQAVSVTNPYPCEEWRSVEDFQGGNKIEVNKNQFALIEGKNKTVSTLVIQAANVSALYKCEAVNKVGRGERVISFHVTRGPEITLQPDLQPTEQESVSLWCTADKSTFENLTWYKLGPQPLPVHVGELPTPVCKNLDTLWKLNATIFSNSTNDILIMELKNASLQDQGDYVCVAQDRKTKKRHCVVRQLTVLERVAPMITGNLENQTTSIGETIEVSCTASGNPPPQIMWFKDNETLVEDSGIVLKDGNRNLTIRRVRKEDEGLYTCQACSVLGCAKVEAFFIIEGAQEKTNLE

Approximately 115-180 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.