Vitronectin 蛋白, Mouse (HEK293, His)

Vitronectin is a key cell adhesion factor in serum and tissues and plays a crucial role in cell interactions by binding to glycosaminoglycans and proteoglycans.It is recognized by specific integrins and serves as an important adhesion molecule, facilitating cell-matrix interactions.Vitronectin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Vitronectin protein, expressed by HEK293 , with C-His labeled tag.

玻连蛋白是一种存在于血清和组织中的关键细胞粘附和扩散因子，在细胞相互作用中发挥着至关重要的作用。它与糖胺聚糖和蛋白聚糖结合，证明了其在分子相互作用中的多功能性。玻连蛋白被特定的整合素识别，作为重要的细胞与基质粘附分子，促进细胞粘附过程。此外，它还充当抑制剂，减轻与末端溶细胞补体途径相关的膜损伤效应。值得注意的是，玻连蛋白与多种分子建立了联系，包括 SERPINE1/PAI1、胰岛素和 C1QBP，展示了其参与多种细胞过程。

1.Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells. When cells are added to mouse Vitronectin coated plates (10 μg/mL and 100 μL/well), > 60% cells will adhere specifically after 30 minutes at 37°C.
2.Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells. When cells are added to mouse Vitronectin coated plates (5 μg/mL and 100 μL/well), approximately is 79.89% adhered specifically after 30 minutes at 37°C.

Mouse

HEK293

C-His

P29788 (D20-K478)

22370  [NCBI]

DQESCKGRCTQGFMASKKCQCDELCTYYQSCCADYMEQCKPQVTRGDVFTMPEDDYWSYDYVEEPKNNTNTGVQPENTSPPGDLNPRTDGTLKPTAFLDPEEQPSTPAPKVEQQEEILRPDTTDQGTPEFPEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDETAVRPGYPKLIQDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPGYPRNISEGFSGIPDNVDAAFALPAHRYSGRERVYFFKGKQYWEYEFQQQPSQEECEGSSLSAVFEHFALLQRDSWENIFELLFWGRSSDGAREPQFISRNWHGVPGKVDAAMAGRIYVTGSLSHSAQAKKQKSKRRSRKRYRSRRGRGHRRSQSSNSRRSSRSIWFSLFSSEESGLGTYNNYDYDMDWLVPATCEPIQSVYFFSGDKYYRVNLRTRRVDSVNPPYPRSIAQYWLGCPTSEK

Approximately 73-90 kDa due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80 or 20 mM PB, 500 mM NaCl , pH 6.5, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.