1. 重组蛋白
  2. Receptor Proteins
  3. VLDLR 蛋白, Human (HEK293, His)

VLDLR 蛋白, Human (HEK293, His)

目录号: HY-P76125
COA 产品使用指南

VLDLR 蛋白是一种多功能受体,通过结合 VLDL 并将其转运到细胞中来介导能量代谢。它与 Reelin/RELN、含 APOE 配体和 clusterin/CLU 相互作用。失活的 VLDLR 与 LRP8 形成寡聚体,但在配体结合后,它会重新排列,通过 DAB1 磷酸化传递细胞外 RELN 信号,调节神经元定位。VLDLR 还充当神经元迁移的停止信号,并在微生物感染期间充当塞姆利基森林病毒的受体。VLDLR 蛋白, Human (HEK293, His) 是重组的 VLDLR 蛋白,由 HEK293 表达,带有 C-His 标签。VLDLR 蛋白, Human (HEK293, His) 全长 770 个氨基酸,糖基化后的分子量为 110-130 kDa。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

VLDLR protein, a versatile receptor, mediates energy metabolism by binding and transporting VLDL into cells. It interacts with Reelin/RELN, APOE-containing ligands, and clusterin/CLU. Inactive VLDLR forms oligomers with LRP8, but upon ligand binding, it rearranges to transmit the extracellular RELN signal via DAB1 phosphorylation, regulating neuron positioning. VLDLR also acts as a stop signal for migrating neurons and serves as a receptor for Semliki Forest virus during microbial infection. VLDLR Protein, Human (HEK293, His) is the recombinant human-derived VLDLR protein, expressed by HEK293, with C-His labeled tag. The total length of VLDLR Protein, Human (HEK293, His) is 770 a.a., with molecular weight (glycosylation form) of 110-130 kDa.

研究背景

VLDLR 蛋白是一种多功能细胞表面受体,通过与 VLDL 结合并促进其内吞转运到细胞中,在能量代谢中发挥关键作用。除了脂质转运之外,VLDLR 还表现出多种结合能力,可与多种分子相互作用,包括 Reelin/RELN、载脂蛋白 E/APOE 含配体和簇蛋白/CLU。在非活性状态下,VLDLR 与 LRP8 形成同源寡聚物或异源寡聚物。配体结合后,同源寡聚物重排成更高级的受体簇,通过与 DAB1 结合将细胞外 RELN 信号传递至细胞内信号通路。这种相互作用会触发 DAB1 的磷酸化,协调准确定位新生成的神经元所需的细胞反应。随后,VLDLR 充当神经元迁移的停止信号,阻止它们进入边缘区。值得注意的是,在微生物感染的情况下,VLDLR 作为塞姆利基森林病毒的受体。

生物活性

Human VLDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of ≤0.72 nM as determined in SPR assay (Biacore T200).

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P98155 (G28--S797)

基因 ID
蛋白结构
N-term
VLDLR (G28--S797)
Accession # P98155
His
C-term
同用名
Very low-density lipoprotein receptor; VLDL-R
氨基酸序列

GRKAKCEPSQFQCTNGRCITLLWKCDGDEDCVDGSDEKNCVKKTCAESDFVCNNGQCVPSRWKCDGDPDCEDGSDESPEQCHMRTCRIHEISCGAHSTQCIPVSWRCDGENDCDSGEDEENCGNITCSPDEFTCSSGRCISRNFVCNGQDDCSDGSDELDCAPPTCGAHEFQCSTSSCIPISWVCDDDADCSDQSDESLEQCGRQPVIHTKCPASEIQCGSGECIHKKWRCDGDPDCKDGSDEVNCPSRTCRPDQFECEDGSCIHGSRQCNGIRDCVDGSDEVNCKNVNQCLGPGKFKCRSGECIDISKVCNQEQDCRDWSDEPLKECHINECLVNNGGCSHICKDLVIGYECDCAAGFELIDRKTCGDIDECQNPGICSQICINLKGGYKCECSRGYQMDLATGVCKAVGKEPSLIFTNRRDIRKIGLERKEYIQLVEQLRNTVALDADIAAQKLFWADLSQKAIFSASIDDKVGRHVKMIDNVYNPAAIAVDWVYKTIYWTDAASKTISVATLDGTKRKFLFNSDLREPASIAVDPLSGFVYWSDWGEPAKIEKAGMNGFDRRPLVTADIQWPNGITLDLIKSRLYWLDSKLHMLSSVDLNGQDRRIVLKSLEFLAHPLALTIFEDRVYWIDGENEAVYGANKFTGSELATLVNNLNDAQDIIVYHELVQPSGKNWCEEDMENGGCEYLCLPAPQINDHSPKYTCSCPSGYNVEENGRDCQSTATTVTYSETKDTNTTEISATSGLVPGGINVTTAVSEVSVPPKGTS

分子量

110-130 kDa (glycosylation)

纯度

Greater than 95% as determined by Tris-Bis PAGE

性状

无菌粉末

组分

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

VLDLR Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
VLDLR Protein, Human (HEK293, His)
目录号:
HY-P76125
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