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  3. Von Willebrand Factor/vWF 蛋白, Human (CHO, His)

Von Willebrand Factor/vWF 蛋白, Human (CHO, His)

目录号: HY-P73541
COA 产品使用指南

Von Willebrand Factor/vWF 蛋白, Human (CHO, His) 是重组的 血管性血友病因子/vWF 蛋白,由 CHO 表达,带有 C-His 标签。Von Willebrand Factor/vWF 蛋白, Human (CHO, His) 全长 2791 个氨基酸,分子量分别约为 ~260 & 350 kDa。

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规格 价格 是否有货 数量
50 μg ¥3780 In-stock
100 μg ¥6430 In-stock
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Von Willebrand Factor/vWF Protein, Human (CHO, His) is the recombinant human-derived Von Willebrand Factor/vWF protein, expressed by CHO , with C-His labeled tag. The total length of Von Willebrand Factor/vWF Protein, Human (CHO, His) is 2791 a.a., with molecular weight of ~260 & 350 kDa, respectively.

生物活性

Immobilized BDD-FVIII at 2 μg/mL (100 μL/well) can bind Recombinant Human vWF Protein (His Tag), with a linear range of 0.16-4.0 μg/mL

种属

Human

表达系统

CHO

标签

C-His

蛋白编号

AAB59458.1 (A23-K2813)

基因 ID
蛋白结构
N-term
vWF (A23-K2813)
Accession # AAB59458.1
His
C-term
同用名
von Willebrand factor; vWF; von Willebrand antigen II; F8VWF
分子量

Approximately 260&350 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Von Willebrand Factor/vWF Protein, Human (CHO, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Von Willebrand Factor/vWF Protein, Human (CHO, His)
目录号:
HY-P73541
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