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  3. WDHD1 蛋白, Human (HEK293, His, Strep)

WDHD1 蛋白, Human (HEK293, His, Strep)

目录号: HY-P701615
产品使用指南

WDHD1 蛋白是核心复制体成分,充当复制起始因子。它直接与 CMG 复合物结合,充当将其他蛋白质招募到复制叉的枢纽。WDHD1 与 POLA1、MCM10、DNA2、CDC45 和 GINS2 相互作用,将其与 CMG 解旋酶复合物连接。WDHD1 蛋白, Human (HEK293, His, Strep) 是重组的 WDHD1 蛋白,由 HEK293 表达,带有 N-10*His, N-Strep 标签。WDHD1 蛋白, Human (HEK293, His, Strep) 全长 1128 个氨基酸。

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描述

WDHD1 Protein is a core replisome component that serves as a replication initiation factor. It directly binds to the CMG complex, acting as a hub to recruit other proteins to the replication fork. WDHD1 interacts with POLA1, MCM10, DNA2, CDC45, and GINS2, linking it to the CMG helicase complex. WDHD1 Protein, Human (HEK293, His, Strep) is the recombinant human-derived WDHD1 protein, expressed by HEK293 , with N-10*His, N-Strep labeled tag. The total length of WDHD1 Protein, Human (HEK293, His, Strep) is 1128 a.a., .

研究背景

WDHD1 是复制体的核心成分,通过直接与 CMG 复合物结合,发挥着作为复制起始因子的关键作用。它的功能不仅限于起始,还充当招募额外蛋白质到复制叉的中心枢纽。在其三聚体形式中,WDHD1 与复制体的各种重要成分相互作用,例如聚合酶 α 催化亚基 POLA1、MCM10、DNA2、CDC45 和 GINS 复合物的 GINS2 亚基。这些相互作用将 WDHD1 与 CMG 解旋酶复合体错综复杂地联系起来,突出了其在协调 DNA 复制所涉及的分子机器的组装和协调方面的重要作用。WDHD1 的多方面相互作用强调了其在分子水平上促进 DNA 复制动态过程的重要性。

种属

Human

表达系统

HEK293

标签

N-10*His;N-Strep

蛋白编号

O75717 (P2-E1129)

基因 ID

11169

蛋白结构
N-term
10*His-Strep
WDHD1 (P2-E1129)
Accession # O75717
Flag
C-term
同用名
WDHD1; WD repeat and HMG-box DNA-binding protein 1; Acidic nucleoplasmic DNA-binding protein 1; And-1
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

WDHD1 Protein, Human (HEK293, His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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WDHD1 Protein, Human (HEK293, His, Strep)
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