WEE2 蛋白, Human (Sf9, His, GST)

WEE2 protein is an oocyte-specific tyrosine kinase that plays an important role in meiosis during prophase I and metaphase II. It maintains meiotic arrest at the germinal vesicle (GV) stage by phosphorylating CDK1 at 'Tyr-15', inhibiting CDK1 activity and preventing reentry. WEE2 Protein, Human (Sf9, His, GST) is the recombinant human-derived WEE2 protein, expressed by Sf9 insect cells , with N-8*His, N-GST labeled tag. The total length of WEE2 Protein, Human (Sf9, His, GST) is 566 a.a., .

WEE2 蛋白是一种卵母细胞特异性蛋白酪氨酸激酶，在 I 前期和 II 中期的减数分裂调节中发挥着关键作用。其重要功能是在生发囊泡 (GV) 阶段维持卵母细胞减数分裂停滞，即在 dictyate 前期 I 的长时间静止期。WEE2 通过在“Tyr-15”处磷酸化 CDK1 来实现这一目标，从而抑制 CDK1 活性，并抑制 CDK1 的活性。防止减数分裂再进入。此外，WEE2 通过在“Tyr-15”处磷酸化 CDK1，有助于促进卵子激活过程中中期 II 的退出。这一作用确保了卵母细胞减数分裂的有序退出，促进原核形成并有助于复杂的生殖过程。

The activity of WEE2 is verified by the ADP-Glo method: Incubate WEE2 protein, ATP, and the substrate at room temperature for 40 minutes, then add the ADP-Glo reagent and read the luminescence signal using the chemiluminescence module of the microplate reader.The specific activity is 1327nM(IC₅₀).

Human

Sf9 insect cells

N-8*His;N-GST

P0C1S8 (D2-H567)

494551

DDKDIDKELRQKLNFSYCEETEIEGQKKVEESREASSQTPEKGEVQDSEAKGTPPWTPLSNVHELDTSSEKDKESPDQILRTPVSHPLKCPETPAQPDSRSKLLPSDSPSTPKTMLSRLVISPTGKLPSRGPKHLKLTPAPLKDEMTSLALVNINPFTPESYKKLFLQSGGKRKIRGDLEEAGPEEGKGGLPAKRCVLRETNMASRYEKEFLEVEKIGVGEFGTVYKCIKRLDGCVYAIKRSMKTFTELSNENSALHEVYAHAVLGHHPHVVRYYSSWAEDDHMIIQNEYCNGGSLQAAISENTKSGNHFEEPKLKDILLQISLGLNYIHNSSMVHLDIKPSNIFICHKMQSESSGVIEEVENEADWFLSANVMYKIGDLGHATSINKPKVEEGDSRFLANEILQEDYRHLPKADIFALGLTIAVAAGAESLPTNGAAWHHIRKGNFPDVPQELSESFSSLLKNMIQPDAEQRPSAAALARNTVLRPSLGKTEELQQQLNLEKFKTATLERELREAQQAQSPQGYTHHGDTGVSGTHTGSRSTKRLVGGKSARSSSFTSGEREPLH

Approximately 90.5 KDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES(pH 7.5), 200 mM NaCl, 20% glycerol, 5 mM DTT, .

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.