WWP2 蛋白, Human (sf9, His-GST)

The WWP2 protein is an E3 ubiquitin protein ligase that coordinates multiple ubiquitination events, including “Lys-63”-linked polyubiquitination of POU5F1, targeting it for proteasomal degradation in embryonic stem cells (ESCs). WWP2 also ubiquitinates EGR2, inhibits activation-induced cell death in T cells, and targets SLC11A2, which is enhanced by NDFIP1 and NDFIP2. WWP2 Protein, Human (sf9, His-GST) is the recombinant human-derived WWP2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

WWP2 蛋白作为 E3 泛素蛋白连接酶，促进泛素从 E2 泛素结合酶转移至其目标底物。值得注意的是，WWP2 通过“Lys-63”连接的缀合参与 POU5F1 的多泛素化，导致 POU5F1 的蛋白酶体降解。在胚胎干细胞 (ESC) 中，这种泛素化被认为在控制 POU5F1 的蛋白水平方面发挥调节作用。此外，WWP2 泛素化 EGR2，促进其蛋白酶体降解，并且在 T 细胞中，这种泛素化与抑制激活诱导的细胞死亡有关。此外，WWP2 以 SLC11A2 为目标进行泛素化，这一过程在 NDFIP1 和 NDFIP2 的存在下得到增强。此外，WWP2 泛素化 RPB1，导致其蛋白酶体降解。这些不同的泛素化事件强调了WWP2在调节参与各种细胞过程的关键蛋白的稳定性和丰度方面的多功能性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His;N-GST

O00308-1 (M1-E870)

11060  [NCBI]

MASASSSRAGVALPFEKSQLTLKVVSAKPKVHNRQPRINSYVEVAVDGLPSETKKTGKRIGSSELLWNEIIILNVTAQSHLDLKVWSCHTLRNELLGTASVNLSNVLKNNGGKMENMQLTLNLQTENKGSVVSGGELTIFLDGPTVDLGNVPNGSALTDGSQLPSRDSSGTAVAPENRHQPPSTNCFGGRSRTHRHSGASARTTPATGEQSPGARSRHRQPVKNSGHSGLANGTVNDEPTTATDPEEPSVVGVTSPPAAPLSVTPNPNTTSLPAPATPAEGEEPSTSGTQQLPAAAQAPDALPAGWEQRELPNGRVYYVDHNTKTTTWERPLPPGWEKRTDPRGRFYYVDHNTRTTTWQRPTAEYVRNYEQWQSQRNQLQGAMQHFSQRFLYQSSSASTDHDPLGPLPPGWEKRQDNGRVYYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESGTKQGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELYFIQDMEILGKVTTHELKEGGESIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKSTIYRHYTKNSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYKSYEQLREKLLYAIEETEGFGQE

Approximately 120 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.