1. 重组蛋白
  2. Enzymes & Regulators
  3. Protein Tyrosine Kinases
  4. Tyrosine-protein Kinase YES
  5. ygiS 蛋白, E.coli (His-SUMO)

ygiS 蛋白, E.coli (His-SUMO)

目录号: HY-P71556
产品使用指南

ygiS蛋白可能是脱氧胆酸转运系统的重要组成部分,并在细胞对胆汁酸去污剂,尤其是脱氧胆酸的反应中发挥重要作用。它的缺失会导致细胞内脱氧胆酸水平升高,从而导致细胞生长减少。ygiS 蛋白, E.coli (His-SUMO) 是重组的 ygiS 蛋白,由 E. coli 表达,带有 N-His, N-SUMO 标签。

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描述

The ygiS protein may be an important component of the deoxycholate transport system and play an important role in cellular responses to bile acid detergents, especially deoxycholate. Its absence leads to increased levels of intracellular deoxycholic acid, resulting in decreased cell growth. ygiS Protein, E.coli (His-SUMO) is the recombinant E. coli-derived ygiS protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

研究背景

ygiS 蛋白可能是脱氧胆酸盐转运系统的重要组成部分,在细胞对胆汁酸去污剂(尤其是脱氧胆酸盐)的反应中发挥着重要作用。在脱氧胆酸存在的情况下,其在 ygiS 缺失突变体中的表达导致细胞内脱氧胆酸水平升高,从而导致细胞生长下降。此外,在脱氧胆酸盐存在的情况下表达升高会导致细胞生长的完全抑制。ygiS 在响应胆汁酸去污剂(例如脱氧胆酸盐)方面的重要性,强调了它可能参与宿主针对胆囊和十二指肠细菌生长的防御机制。

种属

E.coli

表达系统

E. coli

标签

N-His;N-SUMO

蛋白编号

Q46863 (A21-H535)

基因 ID

947140  [NCBI]

蛋白结构
N-term
6*His-SUMO
ygiS (A21-H535)
Accession # Q46863
C-term
同用名
ygiS; b3020; JW2988; Probable deoxycholate-binding periplasmic protein YgiS
氨基酸序列

ADVPANTPLAPQQVFRYNNHSDPGTLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQWSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKATDDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWVVNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIEKSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATTLTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKTAIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKSDSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKLLKPYVGGFPLHNPQDYVYSKELYIKAH

分子量

Approximately 74.4 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ygiS Protein, E.coli (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ygiS Protein, E.coli (His-SUMO)
目录号:
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