ZBED1 蛋白, Human (His)

As an E3-type SUMO ligase, ZBED1 protein catalyzes CHD3/Mi2-α sumoylation, relieves transcriptional repression and promotes the recruitment of RNA polymerase II to gene promoters. This positively regulates H1-5, RPS6, RPL10A and RPL12 transcription, enhancing cell proliferation and growth. ZBED1 Protein, Human (His) is the recombinant human-derived ZBED1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of ZBED1 Protein, Human (His) is 98 a.a., with molecular weight of ~14.0 kDa.

ZBED1 蛋白作为 E3 型小泛素样修饰剂 (SUMO) 连接酶发挥作用，催化 CHD3/Mi2-alpha 的 SUMO 化，导致其从 DNA 解离，随后缓解转录抑制。该事件增强了 RNA 聚合酶 II 向基因启动子的募集，正向调节各种基因的转录，包括 H1-5 和核糖体蛋白，如 RPS6、RPL10A 和 RPL12。ZBED1 协调增强的转录活性可促进细胞增殖，从而促进细胞生长。ZBED1 特异性结合核糖体蛋白基因启动子中的 5'-TGTCG[CT]GA[CT]A-3' 共有序列。在微生物感染的情况下，ZBED1还与人类腺病毒基因启动子结合，在感染早期发挥转录抑制作用并阻碍病毒生长。

Human

E. coli

C-6*His

O96006 (N3-E100)

9189  [NCBI]

NKSLESSQTDLKLVAHPRAKSKVWKYFGFDTNAEGCILQWKKIYCRICMAQIAYSGNTSNLSYHLEKNHPEEFCEFVKSNTEQMREAFATAFSKLKPE

Approximately 14.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.