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ZG16B 蛋白, Human (HEK293, His)

目录号: HY-P700451
COA 产品使用指南

Zymogen granule 蛋白 16 homolog B 是一种分泌型凝集素蛋白,在人类唾液腺组织中高表达。Zymogen granule 蛋白 16 homolog B 与其同源蛋白 ZG16p 具有相同的序列同源性,包含一个 NH2 末端的信号序列,推测相关的凝集素结构域和一个 N- 连接的糖基化位点。ZG16B 蛋白, Human (HEK293, His) 是重组的 ZG16B 蛋白,由 HEK293 表达,带有 C-10*His 标签。

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10 μg ¥780 In-stock
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250 μg ¥8000 In-stock
500 μg ¥14400 In-stock
1 mg ¥25000 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Zymogen granule protein 16 homolog B is a secretory lectin protein that is a highly expressed gene in human salivary gland tissue. Zymogen granule protein 16 homolog B shares sequence homology with its paralog, ZG16p, containing a NH2-terminal signal sequence, putative related lectin domains, and an N-linked glycosylation site. ZG16B Protein, Human (HEK293, His) is the recombinant human-derived ZG16B protein, expressed by HEK293 , with C-10*His labeled tag.

研究背景

Zymogen granule protein 16 homolog B 具有碳水化合物结合活性。Zymogen granule protein 16 homolog B 参与视网膜稳态。Zymogen granule protein 16 homolog B 定位于细胞外泌体[1][2][3]

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Human ZG16B at 2 μg/mL can bind Anti-ZG16B recombinant antibody, the EC50 is ≤95 ng/mL.

  • Immobilized Human ZG16B at 2 μg/mL (100 μL/well) can bind Anti- ZG16B antibody. The ED50 for this effect is 59.89 ng/mL.
种属

Human

表达系统

HEK293

标签

C-10*His

蛋白编号

Q96DA0 (G17-R172)

基因 ID
蛋白结构
N-term
ZG16B (G17-R172)
Accession # Q96DA0
10*His
C-term
同用名
EECP; PAUF; JCLN2; HRPE773; PRO1567
氨基酸序列

GKMYGPGGGKYFSTTEDYDHEITGLRVSVGLLLVKSVQVKLGDSWDVKLGALGGNTQEVTLQPGEYITKVFVAFQAFLRGMVMYTSKDRYFYFGKLDGQISSAYPSQEGQVLVGIYGQYQLLGIKSIGFEWNYPLEEPTTEPPVNLTYSANSPVGR

分子量

Approximately 24-27 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 or PBS, 6%-8% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

ZG16B Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ZG16B Protein, Human (HEK293, His)
目录号:
HY-P700451
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