1. 重组蛋白
  2. Viral Proteins
  3. Zika Virus Proteins
  4. Zika Virus E proteins
  5. Zika virus E/Envelope 蛋白 (Biotinylated, HEK293, His)

Zika virus E/Envelope 蛋白 (Biotinylated, HEK293, His)

目录号: HY-P76129A
产品使用指南

Genome polyprotein 是共价连体的较小蛋白质链,是病毒生存和复制的关键组成部分。Zika virus E/Envelope 蛋白 (Biotinylated, HEK293, His) 是重组的 Zika virus E/Envelope 蛋白,由 HEK293 表达,带有 C-His 标签。

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描述

Genome polyprotein is a smaller protein chain of covalent linkages that is a key component of virus survival and replication. Zika virus E/Envelope Protein (Biotinylated, HEK293, His) is the recombinant virus-derived Zika virus E/Envelope, expressed by HEK293 , with C-His labeled tag.

研究背景

Genome polyprotein 是一系列具有相似或不同功能的蛋白质单元,已被单细胞或多细胞生物体广泛利用,作为无数分子活动的浓缩器。Genome polyprotein 是共价连体的较小蛋白质链,在自然界中作为组织病毒(包括 HIV)蛋白质组的通用手段。Genome polyprotein 作为 NS4B 的信号肽,是 NS4B 抗干扰素活性所必需的。Genome polyprotein 通过干扰宿主 Dicer 抑制 RNA 沉默。Genome polyprotein 可能对病毒出芽起作用。通过 M 外结构域激活线粒体凋亡通路发挥细胞毒性作用。可能显示病毒蛋白活性[1][2]

种属

Virus

表达系统

HEK293

标签

C-His

蛋白编号

ALU33341 (V593-K699)

基因 ID

/

同用名
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-E/Envelope protein (Domain III, His)
氨基酸序列

VSYSLCTAAFTFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGK

分子量

Approximately 13 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Zika virus E/Envelope Protein (Biotinylated, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Zika virus E/Envelope Protein (Biotinylated, HEK293, His)
目录号:
HY-P76129A
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