2. 重组蛋白
  3. Viral Proteins
  4. Zika Virus Proteins
  5. Zika Virus E proteins
  6. Zika virus E/Envelope 蛋白 (T366A, Q367G, W391R, L397R, sf9, His)

Zika virus E/Envelope 蛋白 (T366A, Q367G, W391R, L397R, sf9, His)

目录号: HY-P73534
产品使用指南

Genome polyprotein 是共价连体的较小蛋白质链，是病毒生存和复制的关键组成部分。Zika virus E/Envelope 蛋白 (T366A, Q367G, W391R, L397R, sf9, His) 是重组的 寨卡病毒 E/包膜蛋白，由 Sf9 insect cells 表达，带有 C-His 标签。Zika virus E/Envelope 蛋白 (T366A, Q367G, W391R, L397R, sf9, His) 全长 406 个氨基酸，分子量约为 ~45.6 kDa。

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描述

Genome polyprotein is a smaller protein chain of covalent linkages that is a key component of virus survival and replication. Zika virus E/Envelope Protein (T366A, Q367G, W391R, L397R, sf9, His) is the recombinant Virus-derived Zika virus E/Envelope protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Zika virus E/Envelope Protein (T366A, Q367G, W391R, L397R, sf9, His) is 406 a.a., with molecular weight of ~45.6 kDa.
研究背景

Genome polyprotein 是一系列具有相似或不同功能的蛋白质单元，已被单细胞或多细胞生物体广泛利用，作为无数分子活动的浓缩器。Genome polyprotein 是共价连体的较小蛋白质链，在自然界中作为组织病毒（包括 HIV）蛋白质组的通用手段。Genome polyprotein 作为 NS4B 的信号肽，是 NS4B 抗干扰素活性所必需的。Genome polyprotein 通过干扰宿主 Dicer 抑制 RNA 沉默。Genome polyprotein 可能对病毒出芽起作用。通过 M 外结构域激活线粒体凋亡通路发挥细胞毒性作用。可能显示病毒蛋白活性[1][2]。
种属

Virus
表达系统

Sf9 insect cells
标签

C-His
蛋白编号

ALU33341 (I291-T696,T366A, Q367G, W391R, L397R)
基因 ID

/
同用名
Zika virus (ZIKV) (strain Zika SPH2015) E/Envelope protein (His)
分子量

Approximately 45.6 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Zika virus E/Envelope Protein (T366A, Q367G, W391R, L397R, sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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