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  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. ZRANB1 蛋白, Human (Sf9, His)

ZRANB1 蛋白, Human (Sf9, His)

目录号: HY-P701486
产品使用指南

ZRANB1 是一种泛素硫酯酶，可特异性水解“Lys-29”连接和“Lys-33”连接的双泛素，并对“Lys-63”连接的链具有额外的活性。它通过去泛素化 APC（Wnt 介导的转录抑制剂）来正向调节 Wnt 通路。ZRANB1 蛋白, Human (Sf9, His) 是重组的 ZRANB1 蛋白，由 Sf9 insect cells 表达，带有 C-8*His 标签。ZRANB1 蛋白, Human (Sf9, His) 全长 708 个氨基酸。

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描述

ZRANB1 is a ubiquitin thioesterase that specifically hydrolyzes “Lys-29”-linked and “Lys-33”-linked diubiquitins, with additional activity on “Lys-63”-linked chains. It positively regulates the Wnt pathway by deubiquitinating APC, an inhibitor of Wnt-mediated transcription. ZRANB1 Protein, Human (Sf9, His) is the recombinant human-derived ZRANB1 protein, expressed by Sf9 insect cells , with C-8*His labeled tag. The total length of ZRANB1 Protein, Human (Sf9, His) is 708 a.a., .
研究背景

ZRANB1 作为泛素硫酯酶发挥作用，表现出水解“Lys-29”连接和“Lys-33”连接双泛素的特异性，并且具有裂解“Lys-63”连接链的额外活性，尽管效率较低。值得注意的是，ZRANB1 通过去泛素化 APC 蛋白（Wnt 介导的转录的负调节因子）而充当 Wnt 信号通路的正调节因子。此外，它通过 PIK3C3/VPS34 去泛素化在自噬调节中发挥关键作用，促进自噬体成熟。除了参与细胞内过程之外，ZRANB1 还参与细胞形态和细胞骨架组织的调节，有助于应力纤维动力学和细胞迁移。
种属

Human
表达系统

Sf9 insect cells
标签

C-8*His
蛋白编号

Q9UGI0 (M1-E708)
基因 ID

54764
蛋白结构
N-term
ZRANB1 (M1-E708)
Accession # Q9UGI0
8*His
C-term
同用名
ZRANB1; Ubiquitin thioesterase ZRANB1; TRAF-binding domain-containing protein; hTrabid; Zinc finger Ran-binding domain-containing protein 1
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

ZRANB1 Protein, Human (Sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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