Resazurin  (Synonyms: 刃天青; Diazoresorcinol)

Resazurin (Diazoresorcinol) is a water-soluble, non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal)^[1][2].

Resazurin (Diazoresorcinol 常用于通过其还原为荧光产物 resorufin 测量细菌和真核细胞的活力。在 TSBc 培养基中，Resazurin 在浓度为 44 μM 的情况下接种 F. tularensis LVS 后 24 小时未检测到可存活的细菌。将 Resazurin 的浓度降低至 4.4 μM 仍可导致 F. tularensis LVS 的存活率比单纯培养基减少 10 倍。这两种 Resazurin 处理在 22 小时内均显著减少了存活的 F. tularensis LVS 细菌数量。在感染 HEK293 细胞 22 小时后，Resazurin 处理显著减少了可存活的 F. tularensis LVS 细菌数量^[3]。

使用指南（以下是我们推荐的方案。该方案仅提供指南，应根据您的具体需求进行修改）。
细胞活力^[1][2]：
1. 在 37°C 水浴中解冻 Resazurin 溶液。
2. 将细胞放在 96 孔板上，用 PBS 清洗（避免光照）。
3. 去除 PBS 清洗液，然后加入 500 μL (1μg/mL) 的 Resazurin 溶液。
4. 将板放在培养箱中 30 分钟（培养时间取决于细胞类型和细胞数量）。
5. 使用微孔板读数仪测量 Resorufin 荧光（Ex=530-560 nm，Em=590 nm）。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

229.19

C₁₂H₇NO₄

550-82-3

590

530/560

刃天青

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Silva FSG, et al. Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay. Curr Protoc Toxicol. 2016 May 4;68:2.24.1-2.24.15.  [Content Brief]

  [2]. Rampersad SN. Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays. Sensors (Basel). 2012;12(9):12347-60.  [Content Brief]

  [3]. Schmitt DM, et al. The use of resazurin as a novel antimicrobial agent against Francisella tularensis. Front Cell Infect Microbiol. 2013 Dec 6;3:93.  [Content Brief]