Rf470DL 

Rf470DL is a rotor-fluorogenic D-amino acid (RfDAA). Rf470DL can be used for labeling bacteria (Ex=470 nm, Em=640 nm)^[1].

Rf470DL can realize ash-free imaging of bacterial cell walls^[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[1].
1. RfDAA/FDAA stock solutions are prepared in DMSO at a concentration of 100 mM and stored at -20°C before use.
2.1 For long-pulse labeling of B. subtilis and E. coli, dilute the exponential phase cultures with fresh LB broth containing 1 mM Rf470DL to OD₆₀₀~0.05, and incubate for 1 h.
2.2 Image immediately using a Nikon Ti-E inverted microscopy system without washing and fixation.
3.1 For short-pulse labeling of S. venezuelae, Rf470DL stock solution is added directly to exponential phase cultures to a final concentration of 0.5 mM, followed by incubating at 30 °C with shaking for 15 min.
3.2 Image immediately using a Nikon Ti-E inverted microscopy system without washing and fixation.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

547.07

C₂₆H₃₁ClN₄O₅S

2758364-03-1

640

470

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Yen-Pang Hsu, et al. Fluorogenic D-amino acids enable real-time monitoring of peptidoglycan biosynthesis and high-throughput transpeptidation assays. Nat Chem. 2019 Apr;11(4):335-341.  [Content Brief]