RFRP-3(human) TFA  (Synonyms: Neuropeptide VF(124-131)(human) TFA; Neuropeptide NPVF (human) TFA)

RFRP-3 (Neuropeptide VF(124-131))(human) TFA, a human GnIH peptide homolog, is a potent inhibitor of gonadotropin secretion by inhibiting Ca²⁺?mobilization. RFRP-3(human) is a NPFF1?receptor agonist, it inhibits forskolin-induced production of cAMP with an IC₅₀ of 0.7 nM^[1].

RFRP-3 TFA efficiently inhibits forskolin-induced production of cAMP with an IC₅₀ of 0.7 nM^[1].
Scatchard-plot analysis shows that ¹²⁵I-labelled hRFRP-3 has a single class of high-affinity binding sites for the membrane fractions of CHO cells expressing rat OT7T022, the K_d value and the B_max values are 0.19 nM and 1.3 pM, respectively.
RFRP-3 TFA specifically stimulate cells transfected with a new orphan 7TMR, OT7T022, it binds to OT7T022 as a specific ligand with high affinity (K_d= 0.19 nM)^[1].
RFRP-3 (10^-8?to 10^-14M) has no effect on LH and FSH levels alone, but when it combines with GnRH, LH and FSH secretion is significantly reduced by the combination^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1083.16

C₄₇H₇₃F₃N₁₄O₁₂

VPNLPQRF-NH2

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• [1]. Iain J Clarke, et al. Potent action of RFamide-related peptide-3 on pituitary gonadotropes indicative of a hypophysiotropic role in the negative regulation of gonadotropin secretion. Endocrinology  [Content Brief]