2. GPCR/G Protein Neuronal Signaling
  3. Neuropeptide Y Receptor
  4. RFRP-3(human)

RFRP-3(human)  (Synonyms: Neuropeptide VF(124-131)(human); Neuropeptide NPVF (human))

目录号: HY-P1250 纯度: 99.21%
COA 产品使用指南

摩尔计算器

RFRP-3 (Neuropeptide VF(124-131))(human)，一种人GnIH 肽同源物，是一种促性腺激素分泌抑制 Ca2+ 动员的有效抑制剂。RFRP-3(human) 是一种NPFF1 受体激动剂，它抑制 forskolin (HY-15371) 诱导的 cAMP 生成的 IC50 值为 0.7 nM。

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Custom Peptide Synthesis

RFRP-3(human) Chemical Structure

RFRP-3(human) Chemical Structure

CAS No. : 311309-27-0

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规格 价格 是否有货 数量
1 mg ￥1400
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5 mg ￥4900
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50 mg   询价  
100 mg   询价  

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Customer Review

Other Forms of RFRP-3(human):

RFRP-3(human) 相关抗体

Top Publications Citing Use of Products

查看 Neuropeptide Y Receptor 亚型特异性产品:

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NPY Y1 receptor NPY Y2 receptor NPY Y4 receptor NPY Y5 receptor

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

RFRP-3 (Neuropeptide VF(124-131))(human), a human GnIH peptide homolog, is a potent inhibitor of gonadotropin secretion by inhibiting Ca2+?mobilization. RFRP-3(human) is a NPFF1?receptor agonist, it inhibits forskolin-induced production of cAMP with an IC50 of 0.7 nM[1].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
COS-1 EC50
1.7 nM
Compound: NPVF, VPNLPQRF-NH2
Agonist activity at human NPFF2 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting
Agonist activity at human NPFF2 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting
[PMID: 22708927]
COS-1 EC50
1.7 nM
Compound: NPVF, VPNLPQRF-NH2
Agonist activity at human NPFF1 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting
Agonist activity at human NPFF1 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting
[PMID: 22708927]
体外研究
(In Vitro)

RFRP-3 efficiently inhibits forskolin-induced production of cAMP with an IC50 of 0.7 nM[1].
Scatchard-plot analysis shows that 125I-labelled hRFRP-3 has a single class of high-affinity binding sites for the membrane fractions of CHO cells expressing rat OT7T022, the Kd value and the Bmax values are 0.19 nM and 1.3 pM, respectively.
RFRP-3 specifically stimulate cells transfected with a new orphan 7TMR, OT7T022, it binds to OT7T022 as a specific ligand with high affinity (Kd= 0.19 nM)[1].
RFRP-3 (10-8?to 10-14M) has no effect on LH and FSH levels alone, but when it combines with GnRH, LH and FSH secretion is significantly reduced by the combination[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

RFRP-3(human) 相关抗体:
分子量

969.14

Formula

C45H72N14O10
CAS 号
性状

固体

颜色

White to off-white

Sequence Shortening

VPNLPQRF-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : ≥ 100 mg/mL (103.18 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0318 mL 5.1592 mL 10.3184 mL
5 mM 0.2064 mL 1.0318 mL 2.0637 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

RFRP-3(human) 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.0318 mL 5.1592 mL 10.3184 mL 25.7961 mL
5 mM 0.2064 mL 1.0318 mL 2.0637 mL 5.1592 mL
10 mM 0.1032 mL 0.5159 mL 1.0318 mL 2.5796 mL
15 mM 0.0688 mL 0.3439 mL 0.6879 mL 1.7197 mL
20 mM 0.0516 mL 0.2580 mL 0.5159 mL 1.2898 mL
25 mM 0.0413 mL 0.2064 mL 0.4127 mL 1.0318 mL
30 mM 0.0344 mL 0.1720 mL 0.3439 mL 0.8599 mL
40 mM 0.0258 mL 0.1290 mL 0.2580 mL 0.6449 mL
50 mM 0.0206 mL 0.1032 mL 0.2064 mL 0.5159 mL
60 mM 0.0172 mL 0.0860 mL 0.1720 mL 0.4299 mL
80 mM 0.0129 mL 0.0645 mL 0.1290 mL 0.3225 mL
100 mM 0.0103 mL 0.0516 mL 0.1032 mL 0.2580 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RFRP-3(human)311309-27-0Neuropeptide VF(124-131)(human) Neuropeptide NPVF (human)Neuropeptide Y ReceptorNPY receptorHypothalamicPituitaryAxisLHFSHGnIHhomologInhibitorinhibitorinhibit

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目录号：
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