2. Others
  3. Fluorescent Dye
  4. sBADA TFA

sBADA TFA 是一种有效的绿色荧光染料。sBADA TFA 是一种磺化的 BODIPY-FL 3-amino-D-alanine。sBADA TFA 用于原位标记细菌细胞壁中的肽聚糖。(λex=490 nm，λem=510 nm)。

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sBADA TFA Chemical Structure

sBADA TFA Chemical Structure

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sBADA TFA 的其他形式现货产品:

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sBADA TFA 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

sBADA TFA is a potent green fluorescent dye. sBADA TFA is a sulfonated BODIPY-FL 3-amino-D-alanine. sBADA TFA is used to label peptidoglycans in bacterial cell walls in situ. (λex=490 nm, λem=510 nm)[1].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
sBADA TFA assay[1] (to quantify GAPDH):
1. Wash S. venezuelae cells twice with pre‐warms fresh LB broth (30°C) and collects via centrifuge (7000 g, 2 minutes).
2. S. venezuelae cells are re-suspended in fresh LB broth (30°C) containing 1mM sBADA TFA and cultured for 5 minutes.
3. S. venezuelae cells are treated with 70% ice-cold ethanol and incubates on ice-bath for 1 hour.
4. The fixed cells are then washed twice with 4°C 1x PBS via centrifuge and re‐suspends in 4°C 1x PBS before imaging.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

sBADA TFA 相关抗体:
分子量

594.25

Formula

C19H21BF5N4NaO8S
Emission (Em)

510
Excitation (Ex)

490
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

sBADA TFA 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

sBADAFluorescent Dyegreen fluorescent dyeBODIPY-FL 3-amino-D-alaninesitu tagcell wallpeptidoglycansInhibitorinhibitorinhibit

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目录号：
HY-D1690
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