1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. Thermolysin, Bacillus thermoproteolyticus rokko

Thermolysin, Bacillus thermoproteolyticus rokko  (Synonyms: EC 3.4.24.27; TML)

目录号: HY-P1748
COA 产品使用指南

Thermolysin, Bacillus thermoproteolyticus rokko (EC 3.4.24.27) (TML) 是由革兰氏阳性菌 Bacillus thermoproteolyticus 分泌的耐热的中性金属蛋白酶。Thermolysin 可催化含有疏水残基的肽键水解。

最佳 pH:8 0,在 pH 5.0- 9 5 范围内稳定
最佳温度:70 °C

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Thermolysin, Bacillus thermoproteolyticus rokko Chemical Structure

Thermolysin, Bacillus thermoproteolyticus rokko Chemical Structure

CAS No. : 9073-78-3

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MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Thermolysin, Bacillus thermoproteolyticus rokko (EC 3.4.24.27) (TML) is a thermostable neutral metalloproteinase enzyme secreted by the Gram-positive bacteria Bacillus thermoproteolyticus. Thermolysin catalyzes the hydrolysis of peptide bonds containing hydrophobic residues[1].

Optimal pH: 8.0. Considerably stable from pH 5 to 9.5.
Optimal temperature : 70 °C

体外研究
(In Vitro)

Thermolysin (1 mL 中 100 μg,64°C,4 小时,在分离的淀粉颗粒中) 消化可选择性去除一组 10 至 27 kD 之间的低分子量蛋白质[2].
Thermolysin 可用于水解牛肝肌浆蛋白[3]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Thermolysin(50 mg/kg,口服,一次)可降低 SHR 大鼠的收缩压[4]
Thermolysin 不具有急性毒性,大鼠经口 LD50 超过 18000 mg/kg,小鼠经口 LD50 超过 24000 mg/kg(急性毒性研究)[5]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Spontaneously hypertensive rats (SHR) rats[4]
Dosage: 50 mg/kg
Administration: Oral administration (p.o.), once
Result: Lowered systolic blood pressure in SHR rats, with maximal reduction by 22 mm in systolic blood pressure being observed 6 h after administration.
Clinical Trial
CAS 号
性状

固体

颜色

White to light yellow

中文名称

嗜热菌蛋白酶

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

溶解性数据
细胞实验: 

H2O 中的溶解度 : 1.96 mg/mL (adjust pH to 3 with HCl)

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
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纯度 & 产品资料

参考文献

Thermolysin, Bacillus thermoproteolyticus rokko 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Thermolysin, Bacillus thermoproteolyticus rokko
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