Tris Buffered Saline  (Synonyms: Tris 缓冲盐)

Tris Buffered Saline is a common buffer reagent in molecular biology, consisting of Tris and NaCl. Tris Buffered Saline can adjust pH and ionic strength and is widely used in DNA/RNA extraction, PCR and protein electrophoresis. Tris Buffered Saline can purify and protect nucleic acid materials and improve reaction efficiency and concentration. In protein electrophoresis, Tris Buffered Saline stabilizes gels and maintains pH. Overall, Tris Buffered Saline offers versatility, good solubility, and low toxicity in molecular biology applications.

Tris Buffered Saline 是一种常用的分子生物学实验冲盐，由三甲基甲碱基甲盐（Tris）和钾肥（NaCl）组合而成。它具有调节 pH 值和离子强度、高溶解性与低毒性等特殊点，在 DNA/RNA 提取、PCR、蛋白质电泳等实验中广泛应用。
在 DNA/RNA 提取实验中，Tris Buffered Saline 通常用于 DNA/RNA 的纯化、加速反应过程和平衡确定 DNA/RNA 结构等。Tris Buffered Saline 可以有效保护 DNA/RNA 免受脱氧核糖核酸酶（DNase）和核糖核酸酶（RNase）的降解。
在 PCR 实验中。Tris Buffered Saline 可用于 PCR 反应体系的制备。
Tris Buffered Saline 还可用作凝胶冲液，起到维凝胶稳定性和冲泡电泳液 pH 值的作用。
配制提醒：
MCE 提供片剂 Tris Buffered Saline。可将一片 Tris Buffered Saline 溶于 15mL 去离子水中，得到 0.05M Tris 和 0.15M 氯化钠，在 25℃ 下，pH 7.6。

Tris Buffered Saline 可用于如下研究：
免疫金-银染（IGSS）法研究反应性人扁桃体中的免疫球蛋白^[1]。
研究 α-突触核蛋白的纯化样品的聚集^[2]。
作为蛋白质印迹分析的缓冲液^[3]。
时间分辨荧光免疫分析^[4]。
作为光学显微镜免疫组化的缓冲液^[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

固体

White to off-white

Tris 缓冲盐

Room temperature in continental US; may vary elsewhere.

• [1]. Holgate CS, et al. Immunogold-silver staining: new method of immunostaining with enhanced sensitivity. J Histochem Cytochem. 1983 Jul;31(7):938-44.  [Content Brief]

  [2]. Wood SJ, et al. alpha-synuclein fibrillogenesis is nucleation-dependent. Implications for the pathogenesis of Parkinson's disease. J Biol Chem. 1999 Jul 9;274(28):19509-12.  [Content Brief]

  [3]. Pagès G, et al. Mitogen-activated protein kinases p42mapk and p44mapk are required for fibroblast proliferation. Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8319-23.  [Content Brief]

  [4]. Knight M, et al. Shedding of syncytiotrophoblast microvilli into the maternal circulation in pre-eclamptic pregnancies. Br J Obstet Gynaecol. 1998 Jun;105(6):632-40.  [Content Brief]

  [5]. Maniotis AJ, et al. Vascular channel formation by human melanoma cells in vivo and in vitro: vasculogenic mimicry. Am J Pathol. 1999 Sep;155(3):739-52.  [Content Brief]