2. Cell Cycle/DNA Damage Cytoskeleton Apoptosis
  3. Microtubule/Tubulin Apoptosis
  4. Tubulin polymerization-IN-14

Tubulin polymerization-IN-14 (Compound 20a) 是一种微管蛋白聚合 (tubulin polymerization) 抑制剂，IC50 为 3.15 μM。Tubulin polymerization-IN-14 具有抗血管和抗癌活性，诱导癌细胞凋亡 (apoptosis)。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Tubulin polymerization-IN-14 Chemical Structure

Tubulin polymerization-IN-14 Chemical Structure

CAS No. : 2417134-05-3

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

Tubulin polymerization-IN-14 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Tubulin polymerization-IN-14 (Compound 20a) is a tubulin polymerization inhibitor with an IC50 of 3.15 μM. Tubulin polymerization-IN-14 shows potent anti-vascular and anticancer activities, induces cancer cell apoptosis[1].

IC50 & Target

IC50: 3.15 μM (tubulin polymerization)[1]
细胞效力
(Cellular Effect)
Cell Line Type Value Description References
H22 IC50
0.017 μM
Compound: 20a
Antiproliferative activity against mouse H22 cells assessed as inhibition of cell growth by MTT assay
Antiproliferative activity against mouse H22 cells assessed as inhibition of cell growth by MTT assay
[PMID: 32339853]
HepG2 IC50
0.019 μM
Compound: 20a
Antiproliferative against human HepG2 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay
Antiproliferative against human HepG2 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay
[PMID: 32339853]
体外研究
(In Vitro)

Tubulin polymerization-IN-14 (Compound 20a) binds to the colchicine binding site on tubulin[1].
Tubulin polymerization-IN-14 (0-1 μM; 72 h) inhibits cancer cell growth[1].
Tubulin polymerization-IN-14 (5-20 nM; 48 h) arrests K562 cell cycle at G2/M phase, significantly induces cell apoptosis in K562 cells in a concentration-dependent manner, and induces mitochondrial membrane potential (MMP) collapse and mitochondrial dysfunction in K562 cells[1].
Tubulin polymerization-IN-14 (5-20 nM; 24 h) significantly decreases wound closure and capillary-like tubules formation in a concentration-dependent manner in HUVECs[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Tubulin polymerization-IN-14 相关抗体:

Cell Proliferation Assay[1]

Cell Line: K562 cells
Concentration: 0-1 μM
Incubation Time: 72 h
Result: Showed anti-proliferative activity with an IC50 of 0.01 ± 0.001 μM against K562 cells.

Cell Cytotoxicity Assay[1]

Cell Line: HepG2, HCT-8, MDA-MB-231 and HFL-1 cells
Concentration: 0-1 μM
Incubation Time: 72 h
Result: Showed cytotoxic activities with IC50s of 0.019 ± 0.002, 0.021 ± 0.003, 0.02 ± 0.001 and 0.118 ± 0.007 μM against HepG2, HCT-8, MDA-MB-231 and HFL-1 cells, respectively.

Cell Cycle Analysis[1]

Cell Line: K562 cells
Concentration: 5 nM, 10 nM and 20 nM
Incubation Time: 48 h
Result: 9.40%, 11.54% and 15.28% of cells were arrested at G2/M phase at 5, 10 and 20 nM, respectively.

Apoptosis Analysis[1]

Cell Line: K562 cells
Concentration: 5 nM, 10 nM and 20 nM
Incubation Time: 48 h
Result: Compared to the percentage of apoptosis cells in control group (3.25%), the total percentage of the early (Annexin-Vþ/PI) and late (Annexin-Vþ/PIþ) apoptosis cells were 10.46%, 48.55% and 62.26% after being treated at 5, 10, and 20 nM for 48 h, respectively.

Cell Migration Assay [1]

Cell Line: HUVECs
Concentration: 5 nM, 10 nM and 20 nM
Incubation Time: 24 h
Result: After exposed to compound at 5, 10, and 20 nM for 24 h, cells migrated into 67.6%, 55.3% and 49.2% of the wound area, respectively.
体内研究
(In Vivo)

Tubulin polymerization-IN-14 (Compound 20a) (15 and 30 mg/kg; i.v.; daily for 21 days) displays obvious and dose-dependent antitumor effect with no significant toxicity in the liver tumor allograft mouse model[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Five-week-old male ICR mice, liver tumor allograft model[1]
Dosage: 15 and 30 mg/kg
Administration: Intravenous injection, daily for 21 days
Result: The decrease in tumor weight reached 68.7% at doses of 30 mg/kg per day at 21 days after initiation of treatment as compared to vehicle without obvious loss of body weight.
分子量

290.74

Formula

C15H15ClN2O2
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Tubulin polymerization-IN-14 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tubulin polymerization-IN-142417134-05-3Microtubule/TubulinApoptosisK562anti-vascularanticancertubulin polymerizationInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
Tubulin polymerization-IN-14
目录号：
HY-146211
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z