1. Metabolic Enzyme/Protease
  2. Tyrosinase
  3. Tyrosinase, Mushroom

Tyrosinase, Mushroom  (Synonyms: Polyphenol oxidase)

目录号: HY-125860
COA 产品使用指南 技术支持

Tyrosinase (EC 1.14.18.1) (Polyphenol oxidase) 是控制黑色素产生的限速酶,由 TYR 基因编码。Tyrosinase 主要存在于皮肤黑素细胞合成的黑素体中。

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Tyrosinase, Mushroom Chemical Structure

Tyrosinase, Mushroom Chemical Structure

CAS No. : 9002-10-2

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5 KU ¥500
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Customer Review

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Tyrosinase (EC 1.14.18.1) (Polyphenol oxidase) is a rate-limiting enzyme that controls the production of melanin and is encoded by TYR gene. Tyrosinase is mainly found in melanosomes synthesized by skin melanocytes[1].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
Oocyte IC50
0.0094 μM
Compound: 4
Inhibition of inward ion current elicited by ATP at P2X1 receptor expressed in Xenopus oocytes
Inhibition of inward ion current elicited by ATP at P2X1 receptor expressed in Xenopus oocytes
[PMID: 11462975]
Oocyte IC50
0.14 μM
Compound: 4
Inhibition of inward ion current elicited by ATP was determined at recombinant P2X3 receptor expressed in Xenopus oocytes
Inhibition of inward ion current elicited by ATP was determined at recombinant P2X3 receptor expressed in Xenopus oocytes
[PMID: 11462975]
Oocyte IC50
11.9 μM
Compound: 4
Inhibition of inward ion current elicited by ATP at P2X2 receptor expressed in Xenopus oocytes
Inhibition of inward ion current elicited by ATP at P2X2 receptor expressed in Xenopus oocytes
[PMID: 11462975]
体外研究
(In Vitro)

产品信息
最佳pH: 6.0 - 7.0
理论分子量:128 kDa(四聚体)

酪氨酸酶活性测定
酶液配制
以 1 mg/mL 的浓度溶于 ddH2O 中。再进一步稀释至浓度为 200-400 U/mL。
实验方法
将分光光度计调整到 280 nm 和 25℃。按照以下顺序将样品加入每个比色管中:
1mL 0.5 M Phosphate buffer, pH 6.5
1 mL 0.001 M L-Tyrosine
0.9 mL ddH2O
通过毛细管将氧气充入比色管中 4-5 分钟,使该反应混合物氧化。将比色管置于分光光度计上,记录 4-5 分钟的 A280,记录为空白组吸光度。加入 0.1 mL 适当稀释的酶,记录 10-12 分钟的 A280。
计算
1U=每分钟引起 280 nm 处吸光度增加0.001的酶量。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
CAS 号
性状

固体

颜色

Light brown to brown

中文名称

酪氨酸酶

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

溶解性数据
细胞实验: 

H2O 中的溶解度 : 50 mg/mL (超声助溶)

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
纯度 & 产品资料

参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Tyrosinase, Mushroom
目录号:
HY-125860
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