Vari Fluor 488-Streptavidin 

Vari Fluor 488-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=490 nm/515 nm.

使用方法
1.蛋白处理
使用前，用离心机在 5000× g 下离心 3 min，仅将上清液用于实验，消除蛋白聚集体，减少非特异性背景染色。
2.标记
将 Vari Fluor-Streptavidin 按照 1:500-1:1000稀释后使用，可根据蛋白或抗体的具体情况进行调整。

保存条件
-20℃ 避光保存。

注意事项
1.染料实际含量较少，收到后直接在管中溶解后进行实验。
2.使用前请将产品瞬时离心至管底，再进行后续实验。
3.本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

液体

Light yellow to yellow

515

488

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Nanda JS, et al. Labeling a protein with fluorophores using NHS ester derivitization. Methods Enzymol. 2014;536:87-94.  [Content Brief]