α2-3,6 Neuraminidase, Bifidobacterium infantis  (Synonyms: α2-3,6唾液酸糖苷酶)

α2-3,6 Neuraminidase, Bifidobacterium infantis is a highly specific exoglycosidase that catalyzes the hydrolysis of non-reducing terminal α2-3 and α2-6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. α2-3,6 Neuraminidase does not exhibit activity on α2-8 or branched sialic acids^[1].

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产品信息
本品为大肠杆菌重组表达，C-terminal His Tag，分子量 42 kDa，储存液 20 mM Tris-HCl, pH 7.5，500 mM NaCl，10% glycerol。

使用说明
1.在反应体系中依次加入底物，加入 5 mM MnCl2，调节 pH 为 5.0 混匀，加入适量酶。
2.将样品置于 37℃ 孵育 2-4 小时或者过夜 。
注: 体系放大时候，孵育时间或酶量需要根据实际情况调整，可低速震荡混匀，可以增加转化率。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

9001-67-6

液体

Colorless to light yellow

α2-3,6唾液酸糖苷酶

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Xilong Wang, et al. A novel strategy for quantification of α2,3- and α2,6-linked sialic acids in sialylated glycoproteins. Carbohydr Res. 2023 Sep:531:108892.  [Content Brief]