β-1,4-Galactosyltransferase, neisseria meningitides  (Synonyms: β-1,4-半乳糖基转移酶，来源脑膜炎奈瑟菌)

beta-1,4-Galactosyltransferase (LgtB) (EC 2.4.1.90) (B4GALT1 (LgtB)) is often used in biochemical studies. beta-1,4-Galactosyltransferase (LgtB) catalyzes the reaction involving UDP-galactose and N-acetylglucosamine for the production of galactose beta-1,4-N-acetylglucosamine^[1].

产品信息
该酶用于将Gal以β1,4连接方式连接到以 GlcNAc 或者 Glc 为非还原端的寡糖链上。本品利用大肠杆菌重组表达，N-terminal His Tag，分子量 35 kDa，储存液组分 20 mM Tris-HCl, pH 7.5，0.5 mM NaCl，1 mM DTT。

使用方法
1.按照反应体系加入底物，5 mM MnCl₂,可适当加入缓冲盐 (如 Tris-HCl)，调节pH 为 6.0，加入适量酶进行反应 (根据反应需求)；
2.混匀后，将样品置于 37 ℃ 孵育 2-4 h。

注意事项
体系放大时候，孵育时间或酶量需要根据实际情况调整，可低速震荡混匀。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

9054-94-8

液体

Colorless to light yellow

β-1,4-半乳糖基转移酶，来源脑膜炎奈瑟菌

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Alavi A, et al. Evaluation of beta 1,4-galactosyltransferase in rheumatoid arthritis and its role in the glycosylation network associated with this disease. Glycoconj J. 1995 Jun;12(3):206-10.  [Content Brief]