β-Amyloid (1-42), human  (Synonyms: Amyloid β-peptide (1-42) (human))

β-Amyloid (1-42), human (Amyloid β-peptide (1-42), human) is a 42-amino acid peptide which plays a key role in the pathogenesis of Alzheimer disease^[1][2][3].

β-淀粉样蛋白聚集指南 (以下是我们推荐的方案。此方案仅提供指南，应根据您的具体需要进行修改)。
1. 将固体 Aβ 肽溶解在冷的六氟-2-丙醇 (HFIP) 中) .将肽在室温下孵育至少1小时以建立单体化和结构随机化。
2. 通过蒸发去除HFIP，并将所得肽以薄膜形式储存在 -20或 -80℃。
3. 将得到的膜溶解在5mM的无水DMSO中，然后涡旋稀释成合适的浓度和缓冲液 (无血清和酚红的培养基)。
4. 接下来，将溶液在 4-8°C 下 48 小时。然后将样品在 4-8°C 下以 14000g 离心 10 分钟；可溶性低聚物在上清液中。将上清液稀释 10 -200 倍用于实验。
方法因下游应用而异。
注意：
聚集形式在溶液中不稳定，建议立即使用。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

β-Amyloid (1-42), human TFA 可用于动物建模，构建阿尔兹海默症模型。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

4514.04

C₂₀₃H₃₁₁N₅₅O₆₀S

107761-42-2

固体

White to off-white

Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala

DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

• [1]. Solntseva EI, et al. Impact of amyloid-β peptide (1-42) on voltage-gated ion currents in molluscan neurons. Bull Exp Biol Med. 2011 Oct;151(6):671-4.  [Content Brief]

  [2]. Barucker C, et al. Nuclear translocation uncovers the amyloid peptide Aβ42 as a regulator of gene transcription. J Biol Chem. 2014 Jul 18;289(29):20182-91.  [Content Brief]

  [3]. Stefania Sabella, et al. Capillary electrophoresis studies on the aggregation process of beta-amyloid 1-42 and 1-40 peptides. Electrophoresis. 2004 Oct;25(18-19):3186-94.  [Content Brief]