CU-CPT17e

目录号: HY-101929 纯度: 99.00%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

CU-CPT17e 是一种有效的多 Toll 样受体 (TLR) 激动剂，可激活 TLR3，TLR8 和 TLR9。

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CU-CPT17e Chemical Structure

CAS No. : 2109805-75-4

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5 mg	￥4400	In-stock
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Customer Review

MCE 顾客使用本产品发表的 3 篇科研文献

CU-CPT17e 相关产品

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TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

CU-CPT17e is a potent multi-Toll-like receptor (TLR) agonist that activates TLR3, TLR8, and TLR9.

IC₅₀ & Target^[1]

TLR3

TLR8

TLR9

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	> 100 μM Compound: 17e; CU-CPT17e	Agonist activity at human TLR2 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay Agonist activity at human TLR2 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay	[PMID: 28537730]
HEK293	EC₅₀	> 100 μM Compound: 17e; CU-CPT17e	Agonist activity at human TLR4 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay Agonist activity at human TLR4 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay	[PMID: 28537730]
HEK293	EC₅₀	> 100 μM Compound: 17e; CU-CPT17e	Agonist activity at human TLR5 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay Agonist activity at human TLR5 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay	[PMID: 28537730]
HEK293	EC₅₀	> 100 μM Compound: 17e; CU-CPT17e	Agonist activity at human TLR7 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay Agonist activity at human TLR7 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay	[PMID: 28537730]
HEK293	EC₅₀	13.45 μM Compound: 17e; CU-CPT17e	Agonist activity at human TLR8 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay Agonist activity at human TLR8 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay	[PMID: 28537730]
HEK293	EC₅₀	4.8 μM Compound: 17e; CU-CPT17e	Agonist activity at human TLR3 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay Agonist activity at human TLR3 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay	[PMID: 28537730]
HEK293	EC₅₀	5.66 μM Compound: 17e; CU-CPT17e	Agonist activity at human TLR9 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay Agonist activity at human TLR9 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay	[PMID: 28537730]
HeLa	IC₅₀	15.77 μM Compound: 17e; CU-CPT17e	Antiproliferative activity against human HeLa cells after 48 hrs by WST1 assay Antiproliferative activity against human HeLa cells after 48 hrs by WST1 assay	[PMID: 28537730]
HeLa	IC₅₀	2.71 μM Compound: 17e; CU-CPT17e	Antiproliferative activity against human HeLa cells after 72 hrs by WST1 assay Antiproliferative activity against human HeLa cells after 72 hrs by WST1 assay	[PMID: 28537730]
HeLa	IC₅₀	38.56 μM Compound: 17e; CU-CPT17e	Antiproliferative activity against human HeLa cells after 24 hrs by WST1 assay Antiproliferative activity against human HeLa cells after 24 hrs by WST1 assay	[PMID: 28537730]
HMEC	IC₅₀	> 100 μM Compound: 17e; CU-CPT17e	Antiproliferative activity against HMEC after 24 hrs by WST1 assay Antiproliferative activity against HMEC after 24 hrs by WST1 assay	[PMID: 28537730]
HMEC	IC₅₀	> 100 μM Compound: 17e; CU-CPT17e	Antiproliferative activity against HMEC after 48 hrs by WST1 assay Antiproliferative activity against HMEC after 48 hrs by WST1 assay	[PMID: 28537730]
HMEC	IC₅₀	79.53 μM Compound: 17e; CU-CPT17e	Antiproliferative activity against HMEC after 72 hrs by WST1 assay Antiproliferative activity against HMEC after 72 hrs by WST1 assay	[PMID: 28537730]

体外研究
(In Vitro)

CU-CPT17e 在 TLR3、TLR8 和 TLR9 HEK293 细胞中表现出强烈的 NF-κB 激活，EC₅₀ 值分别为 4.80±0.73、13.5±0.58 和 5.66±0.17 μM。CU-CPT17e 显著提高活性，NF-κB 激活增加 13.9±0.9 倍，EC₅₀ 值为 4.8±0.7 μM。CU-CPT17e 通过触发细胞凋亡和将细胞周期停滞在 S 期来抑制 HeLa 癌细胞的增殖。研究了 CU-CPT17e 在 HeLa 细胞中诱导细胞凋亡。HeLa 细胞在浓度不断增加的 CU-CPT17e 或聚 I:C 或空白对照 (DMSO) 中培养 24 小时。用不同浓度 (10 至 40 μM) 的 CU-CPT17e 处理 24 小时可导致凋亡细胞群升高 10% 至 17%，这比 5 μg/mL 的 poly I:C 更有效。这些结果表明，CU-CPT17e 对 HeLa 细胞的抗增殖活性可能与其直接诱导细胞凋亡的能力有关^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

504.49

Formula

C₂₇H₂₄N₂O₈

CAS 号

2109805-75-4

性状

固体

颜色

Light yellow to yellow

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : 5 mg/mL (9.91 mM; 超声加热助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.9822 mL	9.9110 mL	19.8220 mL
5 mM	0.3964 mL	1.9822 mL	3.9644 mL
10 mM	---	---	---

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

纯度 & 产品资料

纯度: 99.00%

选择批次：

Data Sheet (620 KB) SDS (544 KB)

COA (288 KB) HNMR (270 KB) RP-HPLC (198 KB) MS (69 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Zhang L, et al. Discovery of Small Molecules as Multi-Toll-like Receptor Agonists with Proinflammatory and Anticancer Activities. J Med Chem. 2017 Jun 22;60(12):5029-5044. [Content Brief]

Cell Assay ^[1]	HeLa cells are seeded at a density of 3×10⁵ cells/well in 6-well plates and allowed to attach for 24 h. After treatment of indicated concentrations of CU-CPT17e or poly I:C (5 μg/mL) for another 24 h, cells are harvested with 0.25% trypsin without EDTA and rinsed twice with PBS, then stained using a Annexin V-FITC apoptosis detection kit. Cells are analyzed with a BD Accuri C6 flow cytometer^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Zhang L, et al. Discovery of Small Molecules as Multi-Toll-like Receptor Agonists with Proinflammatory and Anticancer Activities. J Med Chem. 2017 Jun 22;60(12):5029-5044. [Content Brief]

Cell Assay
^[1]

HeLa cells are seeded at a density of 3×10⁵ cells/well in 6-well plates and allowed to attach for 24 h. After treatment of indicated concentrations of CU-CPT17e or poly I:C (5 μg/mL) for another 24 h, cells are harvested with 0.25% trypsin without EDTA and rinsed twice with PBS, then stained using a Annexin V-FITC apoptosis detection kit. Cells are analyzed with a BD Accuri C6 flow cytometer^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Zhang L, et al. Discovery of Small Molecules as Multi-Toll-like Receptor Agonists with Proinflammatory and Anticancer Activities. J Med Chem. 2017 Jun 22;60(12):5029-5044. [Content Brief]

CU-CPT17e 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9822 mL	9.9110 mL	19.8220 mL	49.5550 mL
DMSO	5 mM	0.3964 mL	1.9822 mL	3.9644 mL	9.9110 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CU-CPT17e2109805-75-4Toll-like Receptor (TLR)Inhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

CU-CPT17e

Customer Review

MCE 顾客使用本产品发表的 3 篇科研文献