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  4. Cresyl Violet perchlorate

Cresyl Violet perchlorate  (Synonyms: Oxazine 9 perchlorate)

目录号: HY-101889
产品使用指南

摩尔计算器

Cresyl violet perchlorate 是一种红色荧光染色剂，可用于染色神经元。

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Cresyl Violet perchlorate Chemical Structure

Cresyl Violet perchlorate Chemical Structure

CAS No. : 41830-80-2

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规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

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Cresyl Violet perchlorate 的其他形式现货产品:

Customer Review

Other Forms of Cresyl Violet perchlorate:

Cresyl Violet perchlorate 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Cresyl Violet perchlorate is a red fluorescent stain, which can be used to stain neurons.

体外研究
(In Vitro)

The estimated total number of SG neurons is 27,485±3251 and 26,705±1823 in the PV and Cresyl Violet perchlorate (CV) stained sections, respectively. There is no significant difference between them (p=0.552). Therefore, Cresyl Violet perchlorate staining is simpler and more cost effective when estimates neuronal number. Although PV stains spiral ganglion neurons (SGNs) with a greater intensity and provides a functional status, its tedious protocol limits its use for quantification. Total RC volume is estimated using probe and it is found that an average RC volume of 2.162±0.35 mm3 and 1.82±0.33 mm3 in Cresyl Violet perchlorate stained and PV immunostained sections, respectively. Volume of neurons is estimated using nucleator probe and it is 3487.63±951 μm3 and 3740.1±784 μm3 in CV stained and PV immunostained sections, respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator probe and it is found to be 131.68±50 μm3 and 126.51±33 μm3 in CV stained and PV immunostained sections, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cresyl Violet perchlorate 相关抗体:
分子量

361.74

Formula

C16H12ClN3O5
CAS 号
中文名称

甲酚紫高氯酸盐
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 250 mg/mL (691.10 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.7644 mL 13.8221 mL 27.6442 mL
5 mM 0.5529 mL 2.7644 mL 5.5288 mL
查看完整储备液配制表

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献
Cell Assay
[1]

Cochlear sections containing SGNs are placed in 24 wells plates containing PBS (pH 7.4) and stored at 4°C. The sections are then used for Cresyl Violet perchlorate (CV) and immunohistochemical (IHC) staining. Every 7th section is stained with Cresyl Violet perchlorate (1%), dehydrated with ascending grades of alcohol, cleared with xylene, mounted with DPX and observed under microscope. Approximately 12-13 Cresyl Violet perchlorate stained sections from each specimen are used for stereology. None of these cases show any histopathological changes under the light microscope. Estimation of the total volume of the Rosenthal canal (RC), total number of SGNs (optical fractionator probe) and the volume of the soma and their nucleus (nucleator probe) is done with software[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Cresyl Violet perchlorate 相关分类

完整储备液配制表

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7644 mL 13.8221 mL 27.6442 mL 69.1104 mL
5 mM 0.5529 mL 2.7644 mL 5.5288 mL 13.8221 mL
10 mM 0.2764 mL 1.3822 mL 2.7644 mL 6.9110 mL
15 mM 0.1843 mL 0.9215 mL 1.8429 mL 4.6074 mL
20 mM 0.1382 mL 0.6911 mL 1.3822 mL 3.4555 mL
25 mM 0.1106 mL 0.5529 mL 1.1058 mL 2.7644 mL
30 mM 0.0921 mL 0.4607 mL 0.9215 mL 2.3037 mL
40 mM 0.0691 mL 0.3456 mL 0.6911 mL 1.7278 mL
50 mM 0.0553 mL 0.2764 mL 0.5529 mL 1.3822 mL
60 mM 0.0461 mL 0.2304 mL 0.4607 mL 1.1518 mL
80 mM 0.0346 mL 0.1728 mL 0.3456 mL 0.8639 mL
100 mM 0.0276 mL 0.1382 mL 0.2764 mL 0.6911 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cresyl Violet41830-80-2Oxazine 9甲酚紫高氯酸盐Oxazine9Oxazine-9Fluorescent DyeInhibitorinhibitorinhibit

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