Dolastatin 10 (Synonyms: DLS 10; NSC 376128)

目录号: HY-15580 纯度: 98.11%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Dolastatin 10 (DLS 10) 是一种有效的抗有丝分裂肽，抑制微管蛋白聚合。

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Dolastatin 10 Chemical Structure

CAS No. : 110417-88-4

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规格	价格	是否有货
1 mg	￥1980	In-stock
5 mg	￥5200	In-stock
10 mg	现货	询价
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100 mg		询价

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Dolastatin 10 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

查看 ADC Cytotoxin 亚型特异性产品:

查看所有亚型

Auristatin Calicheamicins Camptothecins Daunorubicins/Doxorubicins Duocarmycins Maytansinoids Pyrrolobenzodiazepines Traditional Cytotoxic Agents

生物活性
纯度 & 产品资料
参考文献

生物活性

Dolastatin 10 (DLS 10) is a potent antimitotic peptide that inhibits tubulin polymerization.

IC₅₀ & Target

Auristatin

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A10	EC₅₀	0.25 nM Compound: 1	Antitumor activity against rat A10 cells assessed as loss of cellular microtubule after 18 hrs by indirect immunofluorescence Antitumor activity against rat A10 cells assessed as loss of cellular microtubule after 18 hrs by indirect immunofluorescence	[PMID: 11473421]
BXPC-3	GI₅₀	0.051 nM Compound: 2	Growth inhibition of human BxPC3 cells after 48 hrs by SRB assay Growth inhibition of human BxPC3 cells after 48 hrs by SRB assay	[PMID: 28926240]
BXPC-3	GI₅₀	0.051 nM Compound: 1	Growth inhibition of human BxPC3 cells after 48 hrs by SRB assay Growth inhibition of human BxPC3 cells after 48 hrs by SRB assay	[PMID: 28895394]
DU-145	GI₅₀	0.007 nM Compound: 2	Growth inhibition of human DU145 cells after 48 hrs by SRB assay Growth inhibition of human DU145 cells after 48 hrs by SRB assay	[PMID: 28926240]
DU-145	GI₅₀	0.007 nM Compound: 1	Growth inhibition of human DU145 cells after 48 hrs by SRB assay Growth inhibition of human DU145 cells after 48 hrs by SRB assay	[PMID: 28895394]
HCT-116	IC₅₀	0.48 nM Compound: 1	Cytotoxicity against human HCT116 cells assessed as reduction in cell viability measured after 48 hrs by ATPlite 1step luminescence assay Cytotoxicity against human HCT116 cells assessed as reduction in cell viability measured after 48 hrs by ATPlite 1step luminescence assay	[PMID: 27380142]
HCT-116	IC₅₀	24 nM Compound: 1	Cytotoxicity against oncogenic KRAS knockout human HCT116 cells expressing wild type KRAS assessed as reduction in cell viability measured after 48 hrs by ATPlite 1step luminescence assay Cytotoxicity against oncogenic KRAS knockout human HCT116 cells expressing wild type KRAS assessed as reduction in cell viability measured after 48 hrs by ATPlite 1step luminescence assay	[PMID: 27380142]
HCT-116	IC₅₀	65 nM Compound: 1	Cytotoxicity against human HIF1alpha and HIF2alpha deficit HCT116 cells assessed as reduction in cell viability measured after 48 hrs by ATPlite 1step luminescence assay Cytotoxicity against human HIF1alpha and HIF2alpha deficit HCT116 cells assessed as reduction in cell viability measured after 48 hrs by ATPlite 1step luminescence assay	[PMID: 27380142]
HT-29	IC₅₀	0.06 nM Compound: 1	Anticancer activity against human HT-29 assessed as reduction in cell viability incubated for 96 hrs by microculture tetrazolium assay Anticancer activity against human HT-29 assessed as reduction in cell viability incubated for 96 hrs by microculture tetrazolium assay	[PMID: 35072477]
KM-20L2	GI₅₀	0.01 nM Compound: 2	Growth inhibition of human KM20L2 cells after 48 hrs by SRB assay Growth inhibition of human KM20L2 cells after 48 hrs by SRB assay	[PMID: 28926240]
KM-20L2	GI₅₀	0.01 nM Compound: 1	Growth inhibition of human KM20L2 cells after 48 hrs by SRB assay Growth inhibition of human KM20L2 cells after 48 hrs by SRB assay	[PMID: 28895394]
L1210	IC₅₀	0.9 nM Compound: 1	Cytotoxicity against mouse L1210 cells assessed as cell growth inhibition measured after 24 hrs Cytotoxicity against mouse L1210 cells assessed as cell growth inhibition measured after 24 hrs	[PMID: 35072477]
MCF7	GI₅₀	0.005 nM Compound: 2	Growth inhibition of human MCF7 cells after 48 hrs by SRB assay Growth inhibition of human MCF7 cells after 48 hrs by SRB assay	[PMID: 28926240]
MCF7	GI₅₀	0.005 nM Compound: 1	Growth inhibition of human MCF7 cells after 48 hrs by SRB assay Growth inhibition of human MCF7 cells after 48 hrs by SRB assay	[PMID: 28895394]
MCF7	IC₅₀	0.083 nM Compound: 4a	Growth inhibition of human MCF7 after 48 hrs by sulforhodamine B method Growth inhibition of human MCF7 after 48 hrs by sulforhodamine B method	[PMID: 16124759]
NCI-H460	GI₅₀	0.229 nM Compound: 2	Growth inhibition of human NCI-H460 cells after 48 hrs by SRB assay Growth inhibition of human NCI-H460 cells after 48 hrs by SRB assay	[PMID: 28926240]
NCI-H460	GI₅₀	0.229 nM Compound: 1	Growth inhibition of human NCI-H460 cells after 48 hrs by SRB assay Growth inhibition of human NCI-H460 cells after 48 hrs by SRB assay	[PMID: 28895394]
SF-268	GI₅₀	0.006 nM Compound: 2	Growth inhibition of human SF268 cells after 48 hrs by SRB assay Growth inhibition of human SF268 cells after 48 hrs by SRB assay	[PMID: 28926240]
SF-268	GI₅₀	0.006 nM Compound: 1	Growth inhibition of human SF268 cells after 48 hrs by SRB assay Growth inhibition of human SF268 cells after 48 hrs by SRB assay	[PMID: 28895394]

体外研究
(In Vitro)

Dolastatin 10 is a unique pentapeptide that isolated from the sea hare Dolabella auricularia. These in vitro data are quite comparable to those of Dolastatin 10 and Auristatin PE, each of which has GI₅₀ values of 10⁻⁵-10⁻⁶ μg/mL (10⁻²-10⁻³ nM) against a similar minipanel of human cell lines^[2]. The antibody-drug conjugate (ADC) comprises the anti-CD30 monoclonal antibody cAC10 conjugated to the cytotoxic agent monomethyl auristatin E (MMAE), a synthetic analog of the tubulin polymerization inhibitor Dolastatin 10^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

785.09

Formula

C₄₂H₆₈N₆O₆S

CAS 号

110417-88-4

性状

固体

颜色

White to off-white

中文名称

尾海兔素10

结构分类

Others

初始来源

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。

溶解性数据

细胞实验：

DMSO 中的溶解度 : ≥ 100 mg/mL (127.37 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.2737 mL	6.3687 mL	12.7374 mL
5 mM	0.2547 mL	1.2737 mL	2.5475 mL
10 mM	0.1274 mL	0.6369 mL	1.2737 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

纯度 & 产品资料

纯度: 98.11%

选择批次：

Data Sheet (601 KB) SDS (394 KB)

COA (202 KB) RP-HPLC (254 KB) MS (70 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Pitot HC, et al. Phase I trial of dolastatin-10 (NSC 376128) in patients with advanced solid tumors. Clin Cancer Res. 1999 Mar;5(3):525-31. [Content Brief]

[2]. Pettit GR, et al. Antineoplastic agents. 592. Highly effective cancer cell growth inhibitory structural modifications ofdolastatin 10. J Nat Prod. 2011 May 27;74(5):962-8. [Content Brief]

[3]. Brentuximab vedotin. Drugs R D. 2011;11(1):85-95. [Content Brief]

Dolastatin 10 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2737 mL	6.3687 mL	12.7374 mL	31.8435 mL
	5 mM	0.2547 mL	1.2737 mL	2.5475 mL	6.3687 mL
	10 mM	0.1274 mL	0.6369 mL	1.2737 mL	3.1843 mL
	15 mM	0.0849 mL	0.4246 mL	0.8492 mL	2.1229 mL
	20 mM	0.0637 mL	0.3184 mL	0.6369 mL	1.5922 mL
	25 mM	0.0509 mL	0.2547 mL	0.5095 mL	1.2737 mL
	30 mM	0.0425 mL	0.2123 mL	0.4246 mL	1.0614 mL
	40 mM	0.0318 mL	0.1592 mL	0.3184 mL	0.7961 mL
	50 mM	0.0255 mL	0.1274 mL	0.2547 mL	0.6369 mL
	60 mM	0.0212 mL	0.1061 mL	0.2123 mL	0.5307 mL
	80 mM	0.0159 mL	0.0796 mL	0.1592 mL	0.3980 mL
	100 mM	0.0127 mL	0.0637 mL	0.1274 mL	0.3184 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dolastatin 10110417-88-4DLS 10 NSC 376128尾海兔素10Dolastatin10Dolastatin-10DLS10DLS-10NSC376128NSC 376128NSC-376128Microtubule/TubulinADC CytotoxinADC PayloadInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Dolastatin 10 (Synonyms: DLS 10; NSC 376128)