Melittin (Synonyms: 蜂毒肽)

目录号: HY-P0233 纯度: 98.47%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Melittin 是一种PLA₂激活剂，可刺激低分子量 PLA₂ 的活性，而不会提高高分子量 PLA₂ 的活性。

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Custom Peptide Synthesis

Melittin Chemical Structure

CAS No. : 20449-79-0

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规格	价格	是否有货
5 mg	￥1500	In-stock
10 mg	￥2600	In-stock
25 mg	￥4700	In-stock
50 mg		询价
100 mg		询价

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Customer Review

Other Forms of Melittin:

Melittin TFA In-stock
Melittin free acid 询价

MCE 顾客使用本产品发表的 11 篇科研文献

: Melittin purchased from MCE. Usage Cited in: J Pharm Pharmacol. 2023 Mar 26;rgad008. [Abstract]; Melittin (2.5, 5, 10 μg/mL; 48 h) downregulates the expression of Bcl-2, and increases the levels of the pro-apoptotic molecules Bax and cyt-c, in SW480 cells.

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Phospholipase A PLD Phospholipase C

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Melittin is a PLA₂ activator, stimulates the activity of the low molecular weight PLA₂, while it does not the increase activity of the high molecular weight PLA₂^[1]^[2].

IC₅₀ & Target

PLA₂^[1]

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
Caco-2	IC₅₀	4.5 μM Compound: Melittin	Cytotoxicity against human Caco2 cells after 1 hr by MTS/PMS assay Cytotoxicity against human Caco2 cells after 1 hr by MTS/PMS assay	[PMID: 24601601]
Erythrocyte	CC₅₀	0.2 μM Compound: Melittin	Hemolytic activity against human RBC assessed as concentration required to cause 50% hemolysis measured after 1 hr Hemolytic activity against human RBC assessed as concentration required to cause 50% hemolysis measured after 1 hr	[PMID: 33397096]
Erythrocyte	IC₅₀	0.96 μM Compound: Melittin	Hemolytic activity against human RBC after 1 hr Hemolytic activity against human RBC after 1 hr	[PMID: 30387611]
HEK-293T	IC₅₀	2.484 μM Compound: Melittin	Cytotoxicity against HEK293T cells assessed as cell death after 18 to 24 hrs by MTT assay Cytotoxicity against HEK293T cells assessed as cell death after 18 to 24 hrs by MTT assay	[PMID: 30742437]
HeLa	IC₅₀	1.9 μM Compound: Melittin	Cytotoxicity against human HeLa cells after 1 hr by MTS/PMS assay Cytotoxicity against human HeLa cells after 1 hr by MTS/PMS assay	[PMID: 24601601]
HepG2	IC₅₀	2.5 μM Compound: Melittin	Cytotoxicity against human HepG2 cells after 1 hr by MTS/PMS assay Cytotoxicity against human HepG2 cells after 1 hr by MTS/PMS assay	[PMID: 24601601]
HUVEC	IC₅₀	13 μM Compound: 1	Antiangiogenic activity against VEGFA-stimulated capillary differentiation in HUVEC after 18 hrs by matrigel assay Antiangiogenic activity against VEGFA-stimulated capillary differentiation in HUVEC after 18 hrs by matrigel assay	[PMID: 23110475]
HUVEC	IC₅₀	18 μM Compound: 1	Antiangiogenic activity against VEGFA-stimulated cell proliferation in HUVEC after 48 hrs by BrdU incorporation assay Antiangiogenic activity against VEGFA-stimulated cell proliferation in HUVEC after 48 hrs by BrdU incorporation assay	[PMID: 23110475]
IEC-6	IC₅₀	2.2 μM Compound: Melittin	Cytotoxicity against rat IEC-6 cells after 1 hr by MTS/PMS assay Cytotoxicity against rat IEC-6 cells after 1 hr by MTS/PMS assay	[PMID: 24601601]
NIH3T3	IC₅₀	6.4 μM Compound: Melittin	Cytotoxicity against mouse NIH/3T3 cells after 1 hr by MTS/PMS assay Cytotoxicity against mouse NIH/3T3 cells after 1 hr by MTS/PMS assay	[PMID: 24601601]
RAW264.7	IC₅₀	1.3 μM Compound: Melittin	Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell growth at pH 6.5 measured after 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell growth at pH 6.5 measured after 24 hrs by MTT assay	[PMID: 35294199]
RAW264.7	IC₅₀	1.94 μM Compound: Melittin	Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell growth at pH 7.4 measured after 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell growth at pH 7.4 measured after 24 hrs by MTT assay	[PMID: 35294199]
RAW264.7	IC₅₀	2.094 μM Compound: Melittin	Cytotoxicity against mouse RAW264.7 cells assessed as cell death after 18 to 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as cell death after 18 to 24 hrs by MTT assay	[PMID: 30742437]

体外研究
(In Vitro)

Melittin, an immunologically related PLA₂ stimulating peptide from bee venom, increases the activity of the high molecular weight enzyme^[1]. Melittin is a cytotoxic peptide from bee venom. Melittin exhibits toxicity against both A2780CR and A2780 cells, with IC₅₀ values of 4.5 and 6.8 μg/mL, respectively. Melittin has natural anti-bacterial, anti-viral, and anti-inflammatory properties. It has also been shown to have diverse anticancer effects in several different cancer cell lines including those of gastric, breast, ovarian, liver, prostate, cervical, and lung origins. The mechanisms by which Melittin, an amphipathic haemolytic peptide, exerts its potential anticancer effects include inhibition of cell proliferation, induction of apoptosis, and direct necrosis. Melittin can also prevent EGF-induced cell invasion through its inhibition of the PI3K/Akt/mTOR signaling pathway, but this is primarily related to breast cancer cells^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

2846.46

Formula

C₁₃₁H₂₂₉N₃₉O₃₁

CAS 号

20449-79-0

性状

固体

颜色

White to off-white

Sequence

Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg-Lys-Arg-Gln-Gln-NH2

Sequence Shortening

GIGAVLKVLTTGLPALISWIKRKRQQ-NH2

中文名称

蜂毒肽；蜂毒素

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

细胞实验：

H₂O 中的溶解度 : ≥ 50 mg/mL (17.57 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.3513 mL	1.7566 mL	3.5131 mL
5 mM	0.0703 mL	0.3513 mL	0.7026 mL
10 mM	0.0351 mL	0.1757 mL	0.3513 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

该产品水溶性佳，请具体参考实测水 / PBS / Saline 中的溶解度数据。

您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。

纯度 & 产品资料

纯度: 99.78%

选择批次：

Data Sheet (601 KB) SDS (480 KB)

COA (280 KB) RP-HPLC (218 KB) LCMS (102 KB) 元素分析报告 (202 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Steiner MR, et al. Responses of purified phospholipases A2 to phospholipase A2 activating protein (PLAP) and Melittin. Biochim Biophys Acta. 1993 Feb 10;1166(1):124-30. [Content Brief]

[2]. Alonezi S, et al. Metabolomic Profiling of the Effects of Melittin on Cisplatin Resistant and Cisplatin Sensitive Ovarian Cancer Cells Using Mass Spectrometry and Biolog Microarray Technology. Metabolites. 2016 Oct 13;6(4). pii: E35. [Content Brief]

Cell Assay ^[2]	Melittin is purified from bee venom by reversed phase liquid chromatography and reconstituted in sterile water to form a stock solution of 1 mg/mL before storage at -20 °C until required for analysis. Cell viability is assessed by an Alamar Blue (AB) cell viability reagent. Both A2780 and A2780CR cells are seeded at 1×10⁴ cells/well in 96-well plates and incubated at 37 °C and 5% CO₂ in a humidified atmosphere for 24 h. After this incubation period, the cells are treated with various concentrations of Melittin ranging from 0.5 to 14 µg/mL in 100 μL of medium, and re-incubated at 37 °C and 5% CO₂ for a further 24 h. Triton X at 1% (v/v) and cell culture media are used as positive and negative controls, respectively. After this, AB is added at a final concentration of 10% (v/v) and the resultant mixture is incubated for a further 4 h at 37 °C and 5% CO₂. Then, the plates are read at an excitation wavelength of 560 nm and the emission at 590 nm is recorded on a SpectraMax M3 microplate reader . Background-corrected fluorescence readings are converted to cell viability data for each test well by expressing them as percentages relative to the mean negative control value^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Steiner MR, et al. Responses of purified phospholipases A2 to phospholipase A2 activating protein (PLAP) and Melittin. Biochim Biophys Acta. 1993 Feb 10;1166(1):124-30. [Content Brief] [2]. Alonezi S, et al. Metabolomic Profiling of the Effects of Melittin on Cisplatin Resistant and Cisplatin Sensitive Ovarian Cancer Cells Using Mass Spectrometry and Biolog Microarray Technology. Metabolites. 2016 Oct 13;6(4). pii: E35. [Content Brief]

Cell Assay
^[2]

Melittin is purified from bee venom by reversed phase liquid chromatography and reconstituted in sterile water to form a stock solution of 1 mg/mL before storage at -20 °C until required for analysis. Cell viability is assessed by an Alamar Blue (AB) cell viability reagent. Both A2780 and A2780CR cells are seeded at 1×10⁴ cells/well in 96-well plates and incubated at 37 °C and 5% CO₂ in a humidified atmosphere for 24 h. After this incubation period, the cells are treated with various concentrations of Melittin ranging from 0.5 to 14 µg/mL in 100 μL of medium, and re-incubated at 37 °C and 5% CO₂ for a further 24 h. Triton X at 1% (v/v) and cell culture media are used as positive and negative controls, respectively. After this, AB is added at a final concentration of 10% (v/v) and the resultant mixture is incubated for a further 4 h at 37 °C and 5% CO₂. Then, the plates are read at an excitation wavelength of 560 nm and the emission at 590 nm is recorded on a SpectraMax M3 microplate reader . Background-corrected fluorescence readings are converted to cell viability data for each test well by expressing them as percentages relative to the mean negative control value^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Steiner MR, et al. Responses of purified phospholipases A2 to phospholipase A2 activating protein (PLAP) and Melittin. Biochim Biophys Acta. 1993 Feb 10;1166(1):124-30. [Content Brief]

[2]. Alonezi S, et al. Metabolomic Profiling of the Effects of Melittin on Cisplatin Resistant and Cisplatin Sensitive Ovarian Cancer Cells Using Mass Spectrometry and Biolog Microarray Technology. Metabolites. 2016 Oct 13;6(4). pii: E35. [Content Brief]

Melittin 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	0.3513 mL	1.7566 mL	3.5131 mL	8.7828 mL
	5 mM	0.0703 mL	0.3513 mL	0.7026 mL	1.7566 mL
	10 mM	0.0351 mL	0.1757 mL	0.3513 mL	0.8783 mL
	15 mM	0.0234 mL	0.1171 mL	0.2342 mL	0.5855 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Melittin20449-79-0蜂毒肽蜂毒素PhospholipaseInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

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上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Melittin (Synonyms: 蜂毒肽)

Customer Review

Other Forms of Melittin:

MCE 顾客使用本产品发表的 11 篇科研文献