2. NF-κB
  3. NF-κB
  4. SN50

SN50是可渗透细胞的 NF-κB 易位抑制剂。

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Custom Peptide Synthesis

SN50 Chemical Structure

SN50 Chemical Structure

CAS No. : 213546-53-3

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
1 mg ￥900
In-stock
5 mg ￥3200
In-stock
10 mg ￥4960
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

SN50 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 35 篇科研文献

WB

    SN50 purchased from MCE. Usage Cited in: Inflammation. 2019 Feb;42(1):319-330.  [Abstract]

    The protein expression of iNOS and COX-2 in TNF-α stimulated RAW264.7 cells with selectively co-cultured with SN50 and SiGDF11, as determined by western blot.

    SN50 purchased from MCE. Usage Cited in: Mol Cell Endocrinol. 2020 Jan 15;500:110641.  [Abstract]

    HUVECs are treated with HG (30 mM), IL-1β (10 ng/mL) and/or SN50 (50 μg/mL), an NF-κB inhibitor. After treatment for 48 h, the protein levels of NF-κB p65 and p-NF-κB p65 in the nucleus of HUVECs were determined using Western blotting. Histone H3 was used for normalization.

    查看 NF-κB 亚型特异性产品:

    查看所有亚型
    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    SN50 is a cell permeable inhibitor of NF-κB translocation.

    IC50 & Target[1]

    NF-κB

     

    体外研究
    (In Vitro)

    Pretreatment with SN50 results in a significant reduction in amount of PI-positive cells at 12, 24, and 48 h time-point post TBI compared with vehicle-treated groups[1]. Topical SN50 suppresses nuclear factor-κB activation in local cells and reduces the incidence of epithelial defects/ulceration in healing corneas. Myofibroblast generation, macrophage invasion, activity of matrix metalloproteinases, basement membrane destruction, and expression of cytokines are all decreased in treated corneas compared with controls[2]. Treating the human gastric cancer cells SGC7901 with SN50 could significantly enhance the effects of LY294002 on inducing cell death after 24 h[3]. SN50 can inhibit translocation of NF-kB and production of inflammatory cytokines that are implicated in lipopolysaccharide (LPS)-induced lung injury[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    SN50 相关抗体:
    体内研究
    (In Vivo)

    Treatment with SN50 accelerates the recovery of motor functional outcome from 1st to 4th day. Animals subjected to SN50 pretreatment demonstrate a significant decrease in the visuospatial learning latencies relative to the control group at 7 and 8 days post-TBI. Pretreatment with SN50 results in a significant reduction of NF-κB p65 protein levels from 6 to 48 h post-TBI and TNF-a protein levels from 12 to 48 h post-TBI[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    2781.50

    Formula

    C129H230N36O29S
    CAS 号
    性状

    固体

    颜色

    White to off-white

    Sequence

    Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Val-Gln-Arg-Lys-Arg-Gln-Lys-Leu-Met-Pro
    Sequence Shortening

    AAVALLPAVLLALLAPVQRKRQKLMP
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : 100 mg/mL (35.95 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    H2O 中的溶解度 : 50 mg/mL (17.98 mM; 超声助溶)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 0.3595 mL 1.7976 mL 3.5952 mL
    5 mM 0.0719 mL 0.3595 mL 0.7190 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 33.33 mg/mL (11.98 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.98%

    COA (186 KB) RP-HPLC (218 KB) LCMS (123 KB) 元素分析报告 (202 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [3]

    SN50 is diluted in distilled sterilization water to create a stock solution. The final concentration of the SN50 solution used is 18 µM. Cell viability is assessed with MTT assay. To determine the effects of SN50 on enhancing the role of LY294002 on SGC7901 cells, cells are plated into 96-well microplates (7×1000 cells/well) and cultured for 24 h. Then LY294002 (50 µM), SN50 (18 µM) and LY294002+SN50 are added to the culture medium and cell viability is assessed with MTT 24 h after drug treatment[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    Mice: SN50 is prepared in saline (total volume: 1 μL, concentration: 0.1 μg/μL). SN50 is administered into the ipsilateral cerebral ventricle 10 min before TBI. After TBI, the bone flap is replaced, the scalp incision is sutured, and then mice are allowed to awaken and returned to their cages. Mice are killed at 1, 6, 12, 24, 48, and 72 h after operation. Loss of plasmalemma integrity is evaluated by intraperitoneal injection of PI 1 h before killing the animal[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    SN50 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 0.3595 mL 1.7976 mL 3.5952 mL 8.9880 mL
    5 mM 0.0719 mL 0.3595 mL 0.7190 mL 1.7976 mL
    10 mM 0.0360 mL 0.1798 mL 0.3595 mL 0.8988 mL
    15 mM 0.0240 mL 0.1198 mL 0.2397 mL 0.5992 mL
    DMSO 20 mM 0.0180 mL 0.0899 mL 0.1798 mL 0.4494 mL
    25 mM 0.0144 mL 0.0719 mL 0.1438 mL 0.3595 mL
    30 mM 0.0120 mL 0.0599 mL 0.1198 mL 0.2996 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    SN50213546-53-3SN 50SN-50NF-κBNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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