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  3. TMRM Perchlorate

TMRM Perchlorate  (Synonyms: T668)

目录号: HY-D0984A 纯度: 99.69%
COA 产品使用指南

罗丹明类染料是一种膜渗透性的阳离子荧光探针,可对线粒体膜电位进行特异性识别,从而附着在线粒体中,并产生明亮荧光,在一定浓度下,罗丹明类染料对细胞具有低毒性,因此普遍用于检测动物细胞、植物细胞以及微生物中的线粒体。

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TMRM Perchlorate Chemical Structure

TMRM Perchlorate Chemical Structure

CAS No. : 115532-50-8

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥551
In-stock
5 mg ¥500
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10 mg ¥800
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50 mg ¥2800
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100 mg ¥4500
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of TMRM Perchlorate:

    TMRM Perchlorate purchased from MCE. Usage Cited in: Endocrinology. 2020 Sep 1;161(9):bqaa119.  [Abstract]

    Mitochondrial membrane potential detected by TMRE. TMRM staining reveals a decreased signal in the Immp2l knockdown granulosa cells compared to control cells, suggesting mitochondrial membrane potential depolarization. Melatonin treatment prevented the membrane depolarization in Immp2l knockdown granulosa cells.
    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms[1].

    体外研究
    (In Vitro)

    1.TMRM 工作液的配制
    1.1 储备液的配制
    取 1mg TMRM 溶于 339 uL DMSO 中,得到 5 mM 储备液。
    1.2 TMRM 工作液的配制
    用无血清细胞培养基或 PBS 稀释储备液,得到 1 -20 μM 工作液。
    注意:请根据实际情况调整 TMRM 工作液的浓度。
    2. 细胞染色
    2.1 悬浮细胞 (6 孔板)
    a.1000g 4℃ 离心 3-5 分钟,弃上清。PBS 洗 2 次,每次 5 分钟。细胞密度为 1×106/mL。
    b. 加入 1 mL 工作液,室温孵育 5-30 分钟。
    c. 400g 4℃ 离心 3-4 分钟,弃上清。
    d. PBS 洗涤 2 次,每次 5 分钟。
    e. 重悬细胞用无血清细胞培养基或 PBS。荧光显微镜或流式细胞仪观察。
    2.2 贴壁细胞
    a. 在无菌盖玻片上培养贴壁细胞。
    b. 从培养基中取出盖玻片并吸出多余的培养基。
    c . 加入 100 μL 工作液,轻摇使之完全覆盖细胞,然后室温孵育 30-60 分钟。
    d. 用培养基洗涤 2 次,每次 5 分钟。荧光显微镜或流式细胞仪观察。
    注:若采用流式细胞仪检测,细胞需重悬后再染色。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    500.93

    Formula

    C25H25ClN2O7

    CAS 号
    性状

    固体

    颜色

    Green to dark green

    Emission (Em)

    576

    Excitation (Ex)

    550

    中文名称

    四甲基罗丹明甲酯高氯酸盐

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

    溶解性数据
    细胞实验: 

    DMSO 中的溶解度 : 41.67 mg/mL (83.19 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 1.9963 mL 9.9814 mL 19.9629 mL
    5 mM 0.3993 mL 1.9963 mL 3.9926 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    纯度 & 产品资料

    纯度: 99.69%

    染色示例
    参考文献
    Cell Assay
    [1]

    Cultures are exposed to Millipore-filtered solutions (0.22 µm) containing TMRM Perchlorate for 1 hr at 37°C (except the experiment involving different durations of exposure to TMRM Perchlorate). After treatment, solutions are removed and growth media reapplied under sterile conditions, and cultures are post-incubated for 18 hours at 37°C (except for the experiment involving analysis at different time points after exposure). Cells are then stained with 2 mg/mL bisbenzimide for 20 min at room temperature. Coverslips are subsequently washed in saline and imaged using 2P microscopy. Apoptotic cells are identified as brightly fluorescent nuclei under UV excitation indicating DNA fragmentation. Cell survivability is calculated as the percentage of live, unstained cells (±SD) in five microscopic fields per treatment[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9963 mL 9.9814 mL 19.9629 mL 49.9072 mL
    5 mM 0.3993 mL 1.9963 mL 3.9926 mL 9.9814 mL
    10 mM 0.1996 mL 0.9981 mL 1.9963 mL 4.9907 mL
    15 mM 0.1331 mL 0.6654 mL 1.3309 mL 3.3271 mL
    20 mM 0.0998 mL 0.4991 mL 0.9981 mL 2.4954 mL
    25 mM 0.0799 mL 0.3993 mL 0.7985 mL 1.9963 mL
    30 mM 0.0665 mL 0.3327 mL 0.6654 mL 1.6636 mL
    40 mM 0.0499 mL 0.2495 mL 0.4991 mL 1.2477 mL
    50 mM 0.0399 mL 0.1996 mL 0.3993 mL 0.9981 mL
    60 mM 0.0333 mL 0.1664 mL 0.3327 mL 0.8318 mL
    80 mM 0.0250 mL 0.1248 mL 0.2495 mL 0.6238 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    产品名称:
    TMRM Perchlorate
    目录号:
    HY-D0984A
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