2. Apoptosis Others
  3. Apoptosis Fluorescent Dye
  4. Umbelliferone

Umbelliferone  (Synonyms: 7-羟基香豆素; 7-Hydroxycoumarin; Hydrangin; NSC 19790)

目录号: HY-N0573 纯度: 99.67%
COA 产品使用指南 技术支持

Umbelliferone (7-Hydroxycoumarin) 是香豆素家族的天然产物，具有口服活性，是一种荧光化合物，可用作防晒剂。 Umbelliferone 诱导 HepG2 细胞的细胞周期停滞，凋亡 (apoptosis) 和 DNA 片段化。Umbelliferone 具有显著的抗癌作用。Umbelliferone 可以减轻过敏性气道炎症的改变特征。Umbelliferone 显示出神经保护作用并穿过血脑屏障。Umbelliferone 在慢性酒精喂养的大鼠中表现出抗炎和抗氧化作用。

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Umbelliferone Chemical Structure

Umbelliferone Chemical Structure

CAS No. : 93-35-6

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥550
In-stock
100 mg ￥500
In-stock
200 mg   询价  
500 mg   询价  

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Customer Review

Other Forms of Umbelliferone:

Umbelliferone 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Umbelliferone (7-Hydroxycoumarin), a natural orally active product of the coumarin family, is a fluorescing compound which can be used as a sunscreen agent. Umbelliferone induces cell cycle arrest, apoptosis and DNA fragmentation in HepG2 cells. Umbelliferone exhibits significant anticancer effects. Umbelliferone attenuates the alteration characteristics of allergic airway inflammation. Umbelliferone displays the neuroprotective effects and cross the blood-brain barrier. Umbelliferone exhibits anti-inflammatory and antioxidant effects in chronic alcohol-fed rats[1][2][3][4].

IC50 & Target

Human Endogenous Metabolite

 

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
> 100 μM
Compound: 6
Cytotoxicity against human A549 cells after 72 hrs by MTT assay
Cytotoxicity against human A549 cells after 72 hrs by MTT assay
[PMID: 21696954]
A549 IC50
21.11 μM
Compound: 7-C
Antiproliferative activity against human A549 cells after 48 hrs by MTT assay
Antiproliferative activity against human A549 cells after 48 hrs by MTT assay
[PMID: 28720504]
BT-549 IC50
1 mM
Compound: 5
Antiproliferative activity against human BT549 cells after 24 hrs by MTT assay
Antiproliferative activity against human BT549 cells after 24 hrs by MTT assay
[PMID: 29144746]
HCT-116 IC50
8.05 μM
Compound: 7
Cytotoxicity against human HCT116 cells after 96 hrs by MTT assay
Cytotoxicity against human HCT116 cells after 96 hrs by MTT assay
[PMID: 23746477]
HEK-293T IC50
189.63 μM
Compound: 7-C
Antiproliferative activity against human 293T cells after 48 hrs by MTT assay
Antiproliferative activity against human 293T cells after 48 hrs by MTT assay
[PMID: 28720504]
HeLa IC50
42.35 μM
Compound: 7-C
Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay
Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay
[PMID: 28720504]
HT-29 IC50
22.67 μM
Compound: 7-C
Antiproliferative activity against human HT-29 cells after 48 hrs by MTT assay
Antiproliferative activity against human HT-29 cells after 48 hrs by MTT assay
[PMID: 28720504]
HT-29 IC50
4.35 μM
Compound: 7
Cytotoxicity against human HT-29 cells after 96 hrs by MTT assay
Cytotoxicity against human HT-29 cells after 96 hrs by MTT assay
[PMID: 23746477]
LNCaP IC50
> 20 μg/mL
Compound: 1
Inhibitory activity against 5-alpha-reductase type 1 in cell culture system using LNCaP cells (androgen-sensitive human prostatic cancer cell line)
Inhibitory activity against 5-alpha-reductase type 1 in cell culture system using LNCaP cells (androgen-sensitive human prostatic cancer cell line)
[PMID: 11527731]
LoVo IC50
97 μM
Compound: 6
Cytotoxicity against human LoVo cells after 72 hrs by MTT assay
Cytotoxicity against human LoVo cells after 72 hrs by MTT assay
[PMID: 21696954]
MCF7 IC50
2.9 μM
Compound: 5
Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay
[PMID: 29144746]
MDA-MB-231 IC50
3.7 μM
Compound: 5
Antiproliferative activity against human MDA-MB-231 cells after 24 hrs by MTT assay
Antiproliferative activity against human MDA-MB-231 cells after 24 hrs by MTT assay
[PMID: 29144746]
MT4 IC50
2341 μM
Compound: 7
Cytotoxicity against human MT4 cells after 96 hrs by MTT assay
Cytotoxicity against human MT4 cells after 96 hrs by MTT assay
[PMID: 23746477]
PC-3 IC50
> 100 μM
Compound: 6
Cytotoxicity against human PC3 cells after 72 hrs by MTT assay
Cytotoxicity against human PC3 cells after 72 hrs by MTT assay
[PMID: 21696954]
SK-MEL-28 IC50
> 100 μM
Compound: 6
Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay
Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay
[PMID: 21696954]
SK-MEL-28 IC50
0.48 μM
Compound: 5
Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
[PMID: 37197457]
SK-MEL3 IC50
1.6 μM
Compound: 5
Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs in presence of Artesunic acid by MTT assay
Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs in presence of Artesunic acid by MTT assay
[PMID: 37197457]
SK-MEL3 IC50
2.1 μM
Compound: 5
Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
[PMID: 37197457]
SMMC-7721 IC50
19.9 μM
Compound: 7-C
Antiproliferative activity against human SMMC7721 cells after 48 hrs by MTT assay
Antiproliferative activity against human SMMC7721 cells after 48 hrs by MTT assay
[PMID: 28720504]
U-373MG ATCC IC50
> 100 μM
Compound: 6
Cytotoxicity against human U373 cells after 72 hrs by MTT assay
Cytotoxicity against human U373 cells after 72 hrs by MTT assay
[PMID: 21696954]
体外研究
(In Vitro)

Umbelliferone (1-50 μM, 12-48 h) 诱导 HepG2 细胞活力降低并呈现剂量和时间依赖性[1]。
Umbelliferone (0-50 μM, 24 h) 导致 HepG2 细胞收缩，膜起泡，核浓缩和凋亡小体形成[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Umbelliferone 相关抗体:

Apoptosis Analysis[1]

Cell Line: HepG2 cells
Concentration: 0, 5, 25 50 μM
Incubation Time: 24 h
Result: Resulted in the appearance of cell shrinkage and membrane blebbing, when compared with the morphology of untreated cells.
At 50 μM almost all the HepG2 cancer cells shrank significantly and no cells with normal morphological features were detected.
Reduced large nuclei.
Induced nuclear condensation and apoptotic body formation.

Cell Cycle Analysis[1]

Cell Line: HepG2 cells
Concentration: 0, 5, 25 50 μM
Incubation Time: 24 h
Result: Increased in the proportion of cells in S phase (45.0, 51.3 and 62.1%, compared with 43.6% in untreated cells) and reduced in the fraction of cells in G1 phase (42.1, 45.6 and 32.2%, compared with 53.2% in the untreated cells).
体内研究
(In Vivo)

Umbelliferone (90 mg/kg, p.o., 每日, 5 天) 可减少受攻击小鼠支气管肺泡灌洗液中的总细胞，中性粒细胞，嗜酸性粒细胞和单核细胞的数量[2]。
Umbelliferone (15-30mg/kg, i.g., 每日, 21 天) 对 CUMS (慢性不可预测的轻度应激) 诱导的抑郁症鼠模型显示出神经保护作用，这与抑制 ROCK/Akt 通路调节的神经元凋亡有关[3]。
Umbelliferone (0.05 g/L, 口服, 每日, 8 周) 通过抑制 TLR4 信号通路和激活抗氧化系统来预防用含5%酒精的利伯德卡里流质饮食诱导大鼠的肝损伤[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male BALB/c mice, 4-6 weeks old received systemic immunization by subcutaneous injection of 10 μg of chicken egg ovalbumin diluted in 2 mg/mL alum followed by a booster injection at day 14. A nasal challenge was performed starting at day 28, by inhalational exposure to aerosolised ovalbumin for 15 min/day, on five consecutive days[2].
Dosage: 30, 60, 90 mg/kg
Administration: p.o., daily, 5 days
Result: Mice sensitized and challenged with ovalbumin have increased the number of inflammatory cells in bronchoalveolar lavage fluid.
Reduced the number of total cells and of neutrophils in the bronchoalveolar lavage fluid.
At 60 and 90 mg/kg, but not at 30 mg/kg, decreased significantly the number of eosinophils found in the bronchoalveolar lavage fluid of challenged mice.
At 60 mg/kg, decreased the number of mononuclear cells.
Caused a reduction in the levels of Th2-associated cytokines (IL-4, IL-5, IL-13).
Animal Model: While the control group of rats without receiving stress treatment had free access to food and water, other groups of rats were subjected to different types of stressors: cage tilting for 24 h, damp sawdust for 24 h, predator sounds, swimming in 4 ℃ cold water for 5min, swimming in 45 ℃ hot water for 5min, fasting for 48 h, water deprivation for 24 h, shaking for 15 min, nip trail for 1 min[3][5].
Dosage: 15,30 mg/kg
Administration: i.g., daily, 21 days
Result: Exhibited an antidepressant-like effect, as sucrose consumption in CUMS rats significantly increased.
Decreased the expression of caspase3 and bax and increased the expression of bcl2 in CA1 region of hippocampus compared to the control group.
Remarkably increased the number of nissl-positive cells.
Down-regulated the levels of inflammatory cytokines IL-1β, IL-6 and TNF-α in hippocampus in the CUMS group versus control group.
Decreased the protein level of ROCK2 and phosphorylation expression of PTEN stimulated by CUMS.
Up-regulated the phosphorylation expressions of Akt and GSK3.
Animal Model: Rats were fed a Liber-Decarli liquid diet containing 5% alcohol for 8 weeks, while normal rats received an isocaloric carbohydrate liquid diet[4].
Dosage: 0.05 g/L
Administration: p.o., daily, 8 weeks
Result: Decreased in serum TNF-α and IL-6 levels and increased IL-10 level.
Decreased the mRNA expressions of LBP, TLR4, NF-κB and TNF-α and IL-6.
Up-regulated SOD and CAT mRNA levels and activity.
Increased H2O2 and GSH levels and decreased hepatic MDA (Malondialdehyde) levels.
分子量

162.14

Formula

C9H6O3
CAS 号
性状

固体

颜色

Light yellow to orange

中文名称

7-羟基香豆素
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 100 mg/mL (616.74 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 6.1674 mL 30.8368 mL 61.6736 mL
5 mM 1.2335 mL 6.1674 mL 12.3347 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 1.43 mg/mL (8.82 mM); 澄清溶液; 超声助溶

    此方案可获得 1.43 mg/mL的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 14.3 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

    生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 1.43 mg/mL (8.82 mM); 澄清溶液; 超声助溶

    此方案可获得 1.43 mg/mL的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 14.3 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

    2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
请输入您的动物体内配方组成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
连续给药周期超过半月以上，请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 99.67%

COA (284 KB) RP-HPLC (242 KB)

产品使用指南 (1538 KB)
参考文献

Umbelliferone 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.1674 mL 30.8368 mL 61.6736 mL 154.1839 mL
5 mM 1.2335 mL 6.1674 mL 12.3347 mL 30.8368 mL
10 mM 0.6167 mL 3.0837 mL 6.1674 mL 15.4184 mL
15 mM 0.4112 mL 2.0558 mL 4.1116 mL 10.2789 mL
20 mM 0.3084 mL 1.5418 mL 3.0837 mL 7.7092 mL
25 mM 0.2467 mL 1.2335 mL 2.4669 mL 6.1674 mL
30 mM 0.2056 mL 1.0279 mL 2.0558 mL 5.1395 mL
40 mM 0.1542 mL 0.7709 mL 1.5418 mL 3.8546 mL
50 mM 0.1233 mL 0.6167 mL 1.2335 mL 3.0837 mL
60 mM 0.1028 mL 0.5139 mL 1.0279 mL 2.5697 mL
80 mM 0.0771 mL 0.3855 mL 0.7709 mL 1.9273 mL
100 mM 0.0617 mL 0.3084 mL 0.6167 mL 1.5418 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Umbelliferone93-35-67-Hydroxycoumarin Hydrangin NSC 197907-羟基香豆素NSC19790NSC 19790NSC-19790ApoptosisFluorescent Dyecell cycleapoptosisDNA fragmentationanticancerInhibitorinhibitorinhibit

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