Histone H3 (acetyl K14) 抗体

Histone H3 (acetyl K14) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 15 kDa, targeting to Histone H3 (acetyl K14). It can be used for WB,ICC/IF,IHC-P,IP,SNAP-ChIP,CUT&Tag-seq assays with tag free, in the background of Human, Mouse, Rat.

Free

Predicted band size: 15 kDa

Protein A affinity purified.

Nucleus. Chromosome.

Non-conjugated

Acetylated

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

SwissProt: P68431 Human;SwissProt: P68433 Mouse;SwissProt: Q6LED0 Rat

WB: 1:500-1:2,000 ;ICC/IF: 1:200-1:1,000 ;IHC-P: 1:50-1:200 ;SNAP-ChIP: 1:100 ;IP: Use at an assay dependent concentration.

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  Western blot analysis of extracts from 293T (lane 1) and Hela (lane 2) and Ramos (lane 3) and Jurkat (lane 4) using Histone H3 (acetyl K14) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ICC/IF, IHC-P, IP, ChIP

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Histone H3.AA range:1-50.