Toll-Like Receptor 4 抗体

Toll-Like Receptor 4 Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 96 kDa, targeting to Toll-Like Receptor 4. It can be used for WB,FC assays with tag free, in the background of Human.

Toll-Like Receptor 4：该基因编码的蛋白质是 Toll 样受体 (TLR) 家族的成员，该家族在病原体识别和先天免疫激活中起着重要作用。TLR 从果蝇到人类高度保守，并具有结构和功能相似性。它们识别在感染因子上表达的病原体相关分子模式，并介导有效免疫力发展所必需的细胞因子的产生。各种 TLR 表现出不同的表达模式。计算机模拟研究发现，表面 TLR4 与严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 的刺突蛋白具有特别强的结合，SARS-CoV-2 是 2019 年冠状病毒病 (COVID-19) 的病原体。该受体还与大多数革兰氏阴性菌中发现的脂多糖 (LPS) 诱导的信号转导事件有关。该基因的突变与 LPS 反应性的差异以及对年龄相关性黄斑变性的易感性有关。已发现该基因编码不同异构体的多个转录变体。[由 RefSeq 提供，2020 年 8 月]

Free

7099

Predicted band size: 96 kDa;Observed band size: 96-130 kDa

affinity purified

Non-conjugated

Unmodified

Immunology

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human

O00206

WB: 1:500-1:1,000 ;FC: 1:50-1:100

• 
  Western blot analysis of extracts from 3T3 (lane 1) and Intestine (lane 2) using Toll-like receptor 4 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, FC

液体

Supplied in 1*PBS (pH 7.3). Preservative: 0.09% sodium azide or 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human TLR4.AA range:669-698 or KLH conjugated synthetic peptide derived from human TLR4: 121-200/839.