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  4. BODIPY-FL

BODIPY-FL 是一种绿色荧光染料，可用于标记探针或引物。BODIPY-FL 荧光可以通过与独特定位的鸟嘌呤相互作用后被淬灭，从而用于定量检测特定 DNA 或 RNA 分子。BODIPY-FL 标记的鞘脂类似物可以用于探究小鼠胚胎干细胞中的鞘脂内化、运输和内吞作用。BODIPY-FL 标记的单萜可快速渗透进细菌、哺乳动物和真菌细胞，可用于检测广谱革兰氏阳性和阴性细菌以及病原真菌的特征。BODIPY-FL 最大吸收波长为 505 nm，最大发射波长为 513 nm。

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BODIPY-FL Chemical Structure

BODIPY-FL Chemical Structure

CAS No. : 165599-63-3

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2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
5 mg ￥260
In-stock
10 mg ￥390
In-stock
25 mg ￥780
In-stock
50 mg ￥1250
In-stock
100 mg ￥1870
In-stock
250 mg ￥3190
In-stock
500 mg   询价  
1 g   询价  

* Please select Quantity before adding items.

Customer Review

BODIPY-FL 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

BODIPY-FL is a green fluorescent dye that can be used to label probes or primers. BODIPY-FL fluorescence can be quenched after interacting with uniquely positioned guanine, making it useful for quantifying specific DNA or RNA molecules. BODIPY-FL-labeled sphingolipid analogs can be used to investigate sphingolipid internalization, transport, and endocytosis in mouse embryonic stem cells. BODIPY-FL-labeled monoterpenes can quickly penetrate bacteria, mammalian, and fungal cells, allowing for the detection of characteristics of a broad spectrum of Gram-positive and Gram-negative bacteria, as well as pathogenic fungi. The maximum absorption wavelength of BODIPY-FL is 505 nm, and the maximum emission wavelength is 513 nm[1][2][3][4].
细胞效力
(Cellular Effect)
Cell Line Type Value Description References
A2780 EC50
> 30 μM
Compound: 7; BODIPY FL
Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
[PMID: 31718946]
A-375 EC50
> 30 μM
Compound: 7; BODIPY FL
Cytotoxicity against human A375 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against human A375 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
[PMID: 31718946]
FaDu EC50
> 30 μM
Compound: 7; BODIPY FL
Cytotoxicity against human FADU cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against human FADU cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
[PMID: 31718946]
HT-29 EC50
> 30 μM
Compound: 7; BODIPY FL
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
[PMID: 31718946]
MCF7 EC50
> 30 μM
Compound: 7; BODIPY FL
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
[PMID: 31718946]
NIH3T3 EC50
> 30 μM
Compound: 7; BODIPY FL
Cytotoxicity against mouse NIH/3T3 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against mouse NIH/3T3 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
[PMID: 31718946]
体外研究
(In Vitro)

BODIPY FL-鞘磷脂染色液的配制[3]
1.1 配制储存液
用氯仿/乙醇溶剂 (19: 1 (v/v)) 配制 1 mM 的 BODIPY FL-鞘磷脂原液。
注：1) BODIPY FL-鞘磷脂储存液转移至琥珀色玻璃圆形样品瓶中并于 -20 ℃ 避光保存。
1.2 配制工作液
1) 将氮气蒸发器的喷嘴放入琥珀色玻璃圆形小瓶中，并将氮气喷向储存液，直至液相完全干燥；再加入 200 µL 无水乙醇，以重构干燥的 BODIPY FL-鞘磷脂粉末。
2) 制备 5 µM 脱脂 BSA 溶液，将适量脱脂 BSA 溶解在 10 mL 含有 10 mM HEPES (HBSS/HEPES 缓冲液) 的 Hanks 缓冲盐溶液中。
3) 将 1) 中制备的 200 μL BODIPY FL-鞘磷脂乙醇溶液加入 2) 中制备的 10 mL 脱脂 BSA 溶液中，在涡流混合器上混合，得到工作液 (5 μM BODIPY FL-鞘磷脂 + 5 μM BSA)。
注：1) 工作液于 -20 ℃ 避光保存。
BODIPY FL-鞘磷脂染色
1) 用 ESGRO 生长培养基培养 mESC 细胞，并将细胞以 5 × 104 个细胞/cm2 的密度接种于培养皿中，37 ℃、5% CO2 培养箱培养过夜。
2) 将过夜细胞培养皿中的生长培养基吸出，并用 HBSS/HEPES 缓冲液洗涤贴壁细胞 3 次。
3) 将 BODIPY FL-鞘磷脂工作液加入培养皿中，完全覆盖细胞生长区域，并在 4 ℃ 下避光孵育细胞 30 分钟。
4) 去除所有工作液，并用 ESGRO 生长培养基洗涤细胞 3 次。
5) 加入 RT ESGRO 生长培养基以覆盖细胞，并在 37 ℃、 5% CO2 的培养箱中避光孵育细胞 30 分钟。
6) 用 ESGRO 生长培养基清洗细胞，用 LSM 780 紫外共聚焦显微镜对染色细胞进行检查和成像。
7) 数据分析 (共聚焦显微镜)：含有高水平 BODIPY FL-鞘脂类似物的细胞器或区域发出红色荧光 (λem ≥ 590 nm)，含有低水平 BODIPY FL-鞘脂类似物的细胞器或区域发出绿色荧光 (λem = 520-590 nm)。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

BODIPY-FL 相关抗体:
分子量

292.09

Formula

C14H15BF2N2O2
CAS 号
性状

固体

颜色

Brown to red

Emission (Em)

516
Excitation (Ex)

495
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 100 mg/mL (342.36 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.4236 mL 17.1180 mL 34.2360 mL
5 mM 0.6847 mL 3.4236 mL 6.8472 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.98%

COA (291 KB) HNMR (245 KB) CNMR (224 KB) RP-HPLC (230 KB)

产品使用指南 (1538 KB)
参考文献

BODIPY-FL 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4236 mL 17.1180 mL 34.2360 mL 85.5901 mL
5 mM 0.6847 mL 3.4236 mL 6.8472 mL 17.1180 mL
10 mM 0.3424 mL 1.7118 mL 3.4236 mL 8.5590 mL
15 mM 0.2282 mL 1.1412 mL 2.2824 mL 5.7060 mL
20 mM 0.1712 mL 0.8559 mL 1.7118 mL 4.2795 mL
25 mM 0.1369 mL 0.6847 mL 1.3694 mL 3.4236 mL
30 mM 0.1141 mL 0.5706 mL 1.1412 mL 2.8530 mL
40 mM 0.0856 mL 0.4280 mL 0.8559 mL 2.1398 mL
50 mM 0.0685 mL 0.3424 mL 0.6847 mL 1.7118 mL
60 mM 0.0571 mL 0.2853 mL 0.5706 mL 1.4265 mL
80 mM 0.0428 mL 0.2140 mL 0.4280 mL 1.0699 mL
100 mM 0.0342 mL 0.1712 mL 0.3424 mL 0.8559 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BODIPY-FL165599-63-3Fluorescent Dyefluorescent dyeprobeprimermonoterpenoidbacteriapathogenic fungiInhibitorinhibitorinhibit

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