1. 诱导疾病模型产品 Immunology/Inflammation
  2. 免疫与炎症疾病模型 Toll-like Receptor (TLR)
  3. 关节炎模型 心肌炎模型
  4. Complete Freund's adjuvant (CFA)

Complete Freund's adjuvant (CFA)  (Synonyms: 完全弗氏佐剂)

目录号: HY-153808
COA 产品使用指南

Complete Freund's adjuvant (CFA) 是因发现者 Jules T. Freund 而获名的、与抗原乳化的免疫佐剂,用于增强动物对抗原 (antigen) 的免疫反应。Complete Freund's adjuvant (CFA) 也是 Th1 型免疫反应的诱导剂和 TLR 配体。Complete Freund's adjuvant (CFA) 包含热杀死的非活性化结核杆菌,并由石蜡油包水乳剂组成。Complete Freund's adjuvant (CFA) 刺激强烈且持久的免疫反应,可用于诱导小鼠的持续性炎症疼痛模型、实验性自身免疫性心肌炎 (EAM) 模型等。Incomplete Freund's adjuvant (IFA) (HY-153808A) 是 Freund's Adjuvant 的另一种类型,刺激的免疫反应较弱。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

Complete Freund's adjuvant (CFA) Chemical Structure

Complete Freund's adjuvant (CFA) Chemical Structure

CAS No. : 9007-81-2

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 mL ¥300
In-stock
2*10 mL ¥500
In-stock
5*10 mL ¥1050
In-stock

* Please select Quantity before adding items.

Customer Review

Other Forms of Complete Freund's adjuvant (CFA):

查看 Toll-like Receptor (TLR) 亚型特异性产品:

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Complete Freund's adjuvant (CFA) is an immunoadjuvant emulsified with antigen by its discoverer Jules T. Freund to enhance an animal's immune response to an antigen. Complete Freund's adjuvant (CFA) is also an inducer of the Th1 immune response and a ligand of TLRs. Complete Freund's adjuvant (CFA) contains heat-killed inactive tuberculosis bacilli and consists of a paraffin oil-in-water emulsion. Complete Freund's adjuvant (CFA) stimulates a strong and durable immune response and can be used to induce persistent inflammatory pain models in mice, experimental autoimmune myocarditis (EAM) models, and more. Incomplete Freund's adjuvant (IFA) (HY-153808A) is another type of Freund's Adjuvant that stimulates a weaker immune response[1][2].

体外研究
(In Vitro)

Complete Freund's adjuvant (CFA) 含有多种微生物 TLR 配体 (如 TLR 2、4 和 9),是 Th1 型免疫反应的有效诱导剂[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Complete Freund's adjuvant (CFA) 诱导实验性自身免疫性心肌炎和神经性疼痛,血浆清除率较低(22%)[1][2][3][4]

1. 诱导实验性自身免疫性心肌炎 (EAM)[1]
致病原理
Complete Freund's adjuvant (CFA) 通过激活免疫系统和增强抗原特异性免疫应答来诱导心肌炎。
具体造模方法:
小鼠:A/J, BALB/c, IL6KO BALB/c • 雄性 • 6-8 周龄
给药方式:5mg/ml • 皮下注射 • 2 次
Note
(1) 在第0天,所有小鼠给予 500 ng 百日咳毒素 (i.p.)
(2) 第 0 天和第 7 天,小鼠皮下注射含有 5 mg/mL 结核分枝杆菌 H37Ra 菌株的 CFA乳化肽。
造模成功指标
组织学分析: 通过组织学和相对心脏重量评估心肌炎严重程度。
分子变化: 检测血清中总IgG,IgG1,IgG2a 和 IgG2b水平,以及 IL-1b 和 IL6 的含量。
相关产品: Incomplete Freund's adjuvant (IFA) (HY-153808A)
拮抗产品: /

2. 诱导神经性疼痛[2][3]
致病原理
Complete Freund's adjuvant (CFA) 在体内释放的炎症介质 TNF-α 和 IL-6 可以激活周围神经末梢并作用于痛觉神经末梢,导致神经性疼痛[2][3]
具体造模方法:
小鼠:C57BL/6J • 雄性 • 8 周龄
给药方式:20 μl • 皮下注射 • 单剂量
Note
(1) 在小鼠后爪足底区注射不稀释的完全弗氏佐剂 (CFA)。
(2) 检测注射 CFA 前及注射后 24、48、72 小时双后爪 PWT、PWL。
造模成功指标
行为观测:检测撤爪阈值 (PWT) 和爪子收缩潜伏期 (PWL)。
相关产品: Galantamine (HY-76299)
拮抗产品Mecamylamine (HY-B1395A)

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: A/J, BALB/c, and IL6KO BALB/c male mice[1]
Dosage:
Administration: Subcutaneous Injectionon (sc); injected at days 0 and 7. EAM severity was analyzed by histology on day 21 (Repeated administration of CFA to animals can cause multiple injection-site effects, the most often observed are on site granulomas and pain)
Result: Increased the relative proportion of CD11b+F4/80−monocytes and CD11b+Gr1+ granulocytes.
Expanded monocytes in the heart and in the spleen and those monocytes in the spleen make less IL-10 as well as more IL-6.
CAS 号
性状

液体

颜色

Colorless to light yellow

中文名称

完全弗氏佐剂

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

纯度 & 产品资料

参考文献
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Complete Freund's adjuvant (CFA)
目录号:
HY-153808
需求量: