Coronaridine

目录号: HY-121118 纯度: 99.23%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Coronaridine，一种生物碱，通过降低 β-catenin 表达来抑制 wnt 信号传导通路。

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Coronaridine Chemical Structure

CAS No. : 467-77-6

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规格	价格	是否有货
1 mg	￥2400	In-stock
5 mg	￥6000	In-stock
10 mg		询价
50 mg		询价

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Customer Review

MCE 顾客使用本产品发表的 1 篇科研文献

•Brain Behav. 2022 Jul 18;e2684. [Abstract]

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生物活性
纯度 & 产品资料
参考文献

生物活性

Coronaridine, an iboga type alkaloid, inhibits the wnt signaling pathway by decreasing β-catenin expression^[1].

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A-431	ED₅₀	19.1 μg/mL Compound: 1	Cytotoxicity against human A431 cells by SRB assay Cytotoxicity against human A431 cells by SRB assay	[PMID: 7853002]
BC1 cell line	ED₅₀	7.5 μg/mL Compound: 1	Cytotoxicity against human BC1 cells by SRB assay Cytotoxicity against human BC1 cells by SRB assay	[PMID: 7853002]
Col2	ED₅₀	> 20 μg/mL Compound: 1	Cytotoxicity against human Col2 cells by SRB assay Cytotoxicity against human Col2 cells by SRB assay	[PMID: 7853002]
DLD-1	IC₅₀	24.4 μM Compound: 3	Cytotoxicity against Wnt-dependent human DLD1 cells assessed as cell viability after 24 hrs by fluorescence assay Cytotoxicity against Wnt-dependent human DLD1 cells assessed as cell viability after 24 hrs by fluorescence assay	[PMID: 26231157]
HCT-116	IC₅₀	11.6 μM Compound: 3	Cytotoxicity against Wnt-dependent human HCT116 cells assessed as cell viability after 24 hrs by fluorescence assay Cytotoxicity against Wnt-dependent human HCT116 cells assessed as cell viability after 24 hrs by fluorescence assay	[PMID: 26231157]
HEK293	IC₅₀	31.7 μM Compound: 3	Cytotoxicity against HEK293 cells assessed as cell viability after 24 hrs by fluorescence assay Cytotoxicity against HEK293 cells assessed as cell viability after 24 hrs by fluorescence assay	[PMID: 26231157]
HEK-293T	IC₅₀	> 40 μM Compound: 3	Cytotoxicity against HEK293T cells assessed as cell viability after 24 hrs by fluorescence assay Cytotoxicity against HEK293T cells assessed as cell viability after 24 hrs by fluorescence assay	[PMID: 26231157]
HeLa	EC₅₀	> 40 μM Compound: 20	Inhibition of lysosomal acidification in human HeLa cells incubated for 3 hrs by LysoTracker Red DND-99 staining based array scan method Inhibition of lysosomal acidification in human HeLa cells incubated for 3 hrs by LysoTracker Red DND-99 staining based array scan method	[PMID: 32356659]
HT	ED₅₀	> 20 μg/mL Compound: 1	Cytotoxicity against human HT cells by SRB assay Cytotoxicity against human HT cells by SRB assay	[PMID: 7853002]
KB	ED₅₀	13.6 μg/mL Compound: 1	Cytotoxicity against human KB cells by SRB assay Cytotoxicity against human KB cells by SRB assay	[PMID: 7853002]
KB-V1	ED₅₀	1.9 μg/mL Compound: 1	Cytotoxicity against human drug-resistant KBV1 cells by SRB assay in presence of vinblastine Cytotoxicity against human drug-resistant KBV1 cells by SRB assay in presence of vinblastine	[PMID: 7853002]
KB-V1	ED₅₀	11.2 μg/mL Compound: 1	Cytotoxicity against human drug-resistant KBV1 cells by SRB assay in absence of vinblastine Cytotoxicity against human drug-resistant KBV1 cells by SRB assay in absence of vinblastine	[PMID: 7853002]
LNCaP	ED₅₀	10.7 μg/mL Compound: 1	Cytotoxicity against human LNCAP cells by SRB assay Cytotoxicity against human LNCAP cells by SRB assay	[PMID: 7853002]
Lu1	ED₅₀	10.9 μg/mL Compound: 1	Cytotoxicity against human Lu1 cells by SRB assay Cytotoxicity against human Lu1 cells by SRB assay	[PMID: 7853002]
P388	ED₅₀	3.8 μg/mL Compound: 1	Cytotoxicity against mouse P388 cells after 48 hrs by SRB assay Cytotoxicity against mouse P388 cells after 48 hrs by SRB assay	[PMID: 7853002]
RAW264.7	IC₅₀	25.5 μM Compound: 10	Anti-inflammatory activity in mouse RAW264.7 cells assessed as LPS-stimulated inhibition of nitric oxide production incubated simultaneously with LPS for 24 hrs by Griess reagent based assay Anti-inflammatory activity in mouse RAW264.7 cells assessed as LPS-stimulated inhibition of nitric oxide production incubated simultaneously with LPS for 24 hrs by Griess reagent based assay	[PMID: 26024020]
RKO	IC₅₀	> 40 μM Compound: 3	Cytotoxicity against Wnt-independent human RKO cells assessed as cell viability after 24 hrs by fluorescence assay Cytotoxicity against Wnt-independent human RKO cells assessed as cell viability after 24 hrs by fluorescence assay	[PMID: 26231157]
SK-MEL-2	ED₅₀	> 20 μg/mL Compound: 1	Cytotoxicity against human SK-MEL-2 cells by SRB assay Cytotoxicity against human SK-MEL-2 cells by SRB assay	[PMID: 7853002]
SW480	IC₅₀	10.4 μM Compound: 3	Cytotoxicity against Wnt-dependent human SW480 cells assessed as cell viability after 24 hrs by fluorescence assay Cytotoxicity against Wnt-dependent human SW480 cells assessed as cell viability after 24 hrs by fluorescence assay	[PMID: 26231157]
U-373MG ATCC	ED₅₀	12 μg/mL Compound: 1	Cytotoxicity against human U373 cells by SRB assay Cytotoxicity against human U373 cells by SRB assay	[PMID: 7853002]
ZR-75-1	ED₅₀	> 20 μg/mL Compound: 1	Cytotoxicity against human ZR-75-1 cells by SRB assay Cytotoxicity against human ZR-75-1 cells by SRB assay	[PMID: 7853002]

体外研究
(In Vitro)

Coronaridine (0-40 μM; 24 hours) is against non-cancer cells with IC₅₀ values >40 μM. It agaisnt wnt-dependent cells with IC₅₀ values of 10.4, 11.6 and 24.4 μM for SW480, HCT116 and DLD1cells, respectively^[1].
Coronaridine (0-40 μM; 24 hours) inhibits β-catenin expression, but the protein levels of p-β--catenin at Ser33, Ser37, and Thr41 and p-β-catenin at Ser 45 [p-b-catenin (S45)] are unchanged^[1].
In whole-cell patch clamp recordings,Catharanthine (1-300 μM) are respectively co-applied with GABA at concentrations corresponding to the EC₃₀ value for each receptor subtype. Both congeners potentiated different GABAARs in a concentration-dependent manner^[2].
At higher concentrations, however, Catharanthine starts to inhibit GABA-activated currents due to the reduced amplitude and rebound current, where the threshold concentration depended on the receptor subtype (e.g., > 30 μM for hα1β2; > 100 μM for hα1β2γ2 and hα2β2γ2). The PAM activity of Catharanthine's are depended on the receptor subtype: hα1β2 (4.6±0.8 μM), >hα2β2γ2 (12.6±3.8 μM), hα1β2γ2 (14.4 ± 4.6 μM)^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

338.44

Formula

C₂₁H₂₆N₂O₂

CAS 号

467-77-6

性状

固体

颜色

White to off-white

结构分类

初始来源

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

细胞实验：

DMSO 中的溶解度 : 50 mg/mL (147.74 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.9547 mL	14.7737 mL	29.5473 mL
5 mM	0.5909 mL	2.9547 mL	5.9095 mL
10 mM	0.2955 mL	1.4774 mL	2.9547 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (3.69 mM); 澄清溶液

此方案可获得 ≥ 1.25 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.72%

选择批次：

Data Sheet (622 KB) SDS (251 KB)

COA (269 KB) HNMR (156 KB) LCMS (74 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Kensuke Ohishi, et al. Coronaridine, an iboga type alkaloid from Tabernaemontana divaricata, inhibits the Wnt signaling pathway by decreasing β-catenin mRNA expression. Bioorg Med Chem Lett. 2015 Sep 15;25(18):3937-40. [Content Brief]

[2]. Hugo R Arias, et al. Coronaridine congeners potentiate GABA A receptors and induce sedative activity in mice in a benzodiazepine-insensitive manner. Prog Neuropsychopharmacol Biol Psychiatry. 2020 Jul 13;101:109930 [Content Brief]

Coronaridine 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9547 mL	14.7737 mL	29.5473 mL	73.8683 mL
	5 mM	0.5909 mL	2.9547 mL	5.9095 mL	14.7737 mL
	10 mM	0.2955 mL	1.4774 mL	2.9547 mL	7.3868 mL
	15 mM	0.1970 mL	0.9849 mL	1.9698 mL	4.9246 mL
	20 mM	0.1477 mL	0.7387 mL	1.4774 mL	3.6934 mL
	25 mM	0.1182 mL	0.5909 mL	1.1819 mL	2.9547 mL
	30 mM	0.0985 mL	0.4925 mL	0.9849 mL	2.4623 mL
	40 mM	0.0739 mL	0.3693 mL	0.7387 mL	1.8467 mL
	50 mM	0.0591 mL	0.2955 mL	0.5909 mL	1.4774 mL
	60 mM	0.0492 mL	0.2462 mL	0.4925 mL	1.2311 mL
	80 mM	0.0369 mL	0.1847 mL	0.3693 mL	0.9234 mL
	100 mM	0.0295 mL	0.1477 mL	0.2955 mL	0.7387 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Coronaridine467-77-6WntNon-cancercancersedativeInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Coronaridine

Customer Review

MCE 顾客使用本产品发表的 1 篇科研文献