2. Immunology/Inflammation Metabolic Enzyme/Protease
  3. STING Endogenous Metabolite
  4. Cyclic-di-GMP disodium

Cyclic-di-GMP disodium  (Synonyms: c-di-GMP disodium; cyclic diguanylate disodium; 5GP-5GP disodium)

目录号: HY-110382 纯度: 99.23%
COA 产品使用指南

摩尔计算器

Cyclic-di-GMP disodium 是一种 STING 激动剂，也是细菌第二信使，协调细菌生长和行为的不同方面，包括运动能力、毒力、生物膜的形成和细胞周期的进展。Cyclic-di-GMP disodium 具有抗癌细胞增殖活性，还可诱导 CD4 受体表达升高和细胞周期停滞。Cyclic-di-GMP disodium 可用于癌症的研究。

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Cyclic-di-GMP disodium Chemical Structure

Cyclic-di-GMP disodium Chemical Structure

CAS No. : 2222132-40-1

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1 mg ￥2500
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5 mg ￥4500
In-stock
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100 mg   询价  

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Customer Review

Other Forms of Cyclic-di-GMP disodium:

Cyclic-di-GMP disodium 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Cyclic-di-GMP disodium is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP disodium has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP disodium can be used in cancer research[1][2][3][4].

IC50 & Target
体外研究
(In Vitro)

Cyclic-di-GMP disodium (0.5-50 μM; 5 days) inhibits proliferation of human colon cancer cells[1].
? Cyclic-di-GMP disodium (0.5-50 μM; 5 days) specifically elevates CD4 expression in Jurkat cells[2].
? Cyclic-di-GMP disodium (0.5-50 μM; 5 days) induces cell cycle arrest at the S-phase in Jurkat cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cyclic-di-GMP disodium 相关抗体:

Cell Proliferation Assay[1]

Cell Line: H508 cells
Concentration: 0.5-50 µM
Incubation Time: 5 days
Result: Reduced basal H508 cell proliferation by approx 15%, even inhibited acetylcholine- and EGF-induced cell proliferation.

Cell Viability Assay[2]

Cell Line: Jurkat cells
Concentration: 50 µM
Incubation Time: 24 h
Result: Specifically induced of CD4 (no effect on the expression of CD8), with a 6.3-fold upregulation over control and in a dose-dependent manner.

Cell Cycle Analysis[2]

Cell Line: Jurkat cells
Concentration: 50 µM
Incubation Time: 24 h
Result: Increased the percentage of cells in S-phase by 79%, with almost complete disappearance of G2/M-phase cells which decreased by 93%.
体内研究
(In Vivo)

Cyclic-di-GMP disodium (100 μg/per; i.v.; two sequential vaccinations 9 days apart) enhances TriVax-induced immune responses to melanoma in mice and further increased the anti-tumor effects of TriVax[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 (B6) mice (8- to 10-week-old)[3].
Dosage: 100 µg/per
Administration: Intravenous injection; two sequential vaccinations 9 days apart; combine with TriVax.
Result: Significantly higher numbers of antigen-specific CD8 T cells when combined with TriVax. (TriVax consisted of a mixture of 120 μg Pam-hgp100, 100 µg hgp100 or 100 µg Ova, 50 or 25 μg anti-CD40 antibody, and 25 μg Poly-IC).
Enhanced the anti-tumor activity of TriVax.
分子量

734.37

Formula

C20H22N10Na2O14P2
CAS 号
性状

固体

颜色

Off-white to light yellow

结构分类
初始来源
运输条件

Shipping with dry ice.
储存方式

-80°C, protect from light, stored under nitrogen
溶解性数据
细胞实验： 

H2O 中的溶解度 : 160 mg/mL (217.87 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.3617 mL 6.8086 mL 13.6171 mL
5 mM 0.2723 mL 1.3617 mL 2.7234 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months (protect from light, stored under nitrogen)。-80°C 储存时，请在6个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

  • 方案 一

    请依序添加每种溶剂： PBS

    Solubility: 50 mg/mL (68.09 mM); 澄清溶液; 超声助溶

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料
参考文献

Cyclic-di-GMP disodium 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months (protect from light, stored under nitrogen)。-80°C 储存时，请在6个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.3617 mL 6.8086 mL 13.6171 mL 34.0428 mL
5 mM 0.2723 mL 1.3617 mL 2.7234 mL 6.8086 mL
10 mM 0.1362 mL 0.6809 mL 1.3617 mL 3.4043 mL
15 mM 0.0908 mL 0.4539 mL 0.9078 mL 2.2695 mL
20 mM 0.0681 mL 0.3404 mL 0.6809 mL 1.7021 mL
25 mM 0.0545 mL 0.2723 mL 0.5447 mL 1.3617 mL
30 mM 0.0454 mL 0.2270 mL 0.4539 mL 1.1348 mL
40 mM 0.0340 mL 0.1702 mL 0.3404 mL 0.8511 mL
50 mM 0.0272 mL 0.1362 mL 0.2723 mL 0.6809 mL
60 mM 0.0227 mL 0.1135 mL 0.2270 mL 0.5674 mL
80 mM 0.0170 mL 0.0851 mL 0.1702 mL 0.4255 mL
100 mM 0.0136 mL 0.0681 mL 0.1362 mL 0.3404 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cyclic-di-GMP disodium2222132-40-1c-di-GMP disodiumcyclic diguanylate disodium5GP-5GP disodiumSTINGEndogenous MetaboliteStimulator of Interferon GenesTMEM173MITAERISMPYSimmunotherapymalignant melanomaH508Jurkat cellscancerCD4Inhibitorinhibitorinhibit

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