2. Immunology/Inflammation Metabolic Enzyme/Protease
  3. STING Endogenous Metabolite
  4. Cyclic-di-GMP

Cyclic-di-GMP  (Synonyms: c-di-GMP; cyclic diguanylate; 5GP-5GP)

目录号: HY-107780
COA 产品使用指南

摩尔计算器

Cyclic-di-GMP 是一种 STING 激动剂，也是细菌第二信使，协调细菌生长和行为的不同方面，包括运动能力、毒力、生物膜的形成和细胞周期的进展。Cyclic-di-GMP 具有抗癌细胞增殖活性，还可诱导 CD4 受体表达升高和细胞周期停滞。Cyclic-di-GMP 可用于癌症的研究。

该游离形式化合物不稳定，推荐具有相同生物学活性的稳定盐形式 Cyclic-di-GMP diammonium 和 Cyclic-di-GMP disodium。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Cyclic-di-GMP Chemical Structure

Cyclic-di-GMP Chemical Structure

CAS No. : 61093-23-0

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规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Cyclic-di-GMP 的其他形式现货产品:

Customer Review

Other Forms of Cyclic-di-GMP:

Cyclic-di-GMP 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Cyclic-di-GMP is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP can be used in cancer research[1][2][3][4].

IC50 & Target
体外研究
(In Vitro)

Cyclic-di-GMP (0.5-50 µM; 5 days) inhibits proliferation of human colon cancer cells[1].
Cyclic-di-GMP (0.5-50 µM; 5 days) specifically elevates CD4 expression in Jurkat cells[2].
Cyclic-di-GMP (0.5-50 µM; 5 days) induces cell cycle arrest at the S-phase in Jurkat cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cyclic-di-GMP 相关抗体:

Cell Proliferation Assay[1]

Cell Line: H508 cells
Concentration: 0.5-50 µM
Incubation Time: 5 days
Result: Reduced basal H508 cell proliferation by approx 15%, even inhibited acetylcholine- and EGF-induced cell proliferation.

Cell Viability Assay[2]

Cell Line: Jurkat cells
Concentration: 50 µM
Incubation Time: 24 h
Result: Specifically induced of CD4 (no effect on the expression of CD8), with a 6.3-fold upregulation over control and in a dose-dependent manner.

Cell Cycle Analysis[2]

Cell Line: Jurkat cells
Concentration: 50 µM
Incubation Time: 24 h
Result: Increased the percentage of cells in S-phase by 79%, with almost complete disappearance of G2/M-phase cells which decreased by 93%.
体内研究
(In Vivo)

Cyclic-di-GMP (100 µg/per; i.v.; two sequential vaccinations 9 days apart) enhances TriVax-induced immune responses to melanoma in mice and further increased the anti-tumor effects of TriVax[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 (B6) mice (8- to 10-week-old)[3].
Dosage: 100 µg/per
Administration: Intravenous injection; two sequential vaccinations 9 days apart; combine with TriVax.
Result: Significantly higher numbers of antigen-specific CD8 T cells when combined with TriVax. (TriVax consisted of a mixture of 120 μg Pam-hgp100, 100 µg hgp100 or 100 µg Ova, 50 or 25 μg anti-CD40 antibody, and 25 μg Poly-IC).
Enhanced the anti-tumor activity of TriVax.
分子量

690.41

Formula

C20H24N10O14P2
CAS 号
性状

固体

颜色

White to off-white

结构分类
初始来源

bacterial species
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性数据
细胞实验： 

H2O 中的溶解度 : ≥ 25 mg/mL (36.21 mM)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.4484 mL 7.2421 mL 14.4841 mL
5 mM 0.2897 mL 1.4484 mL 2.8968 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: 98.18%

COA (198 KB) HNMR (196 KB) LCMS (774 KB)

产品使用指南 (1538 KB)
参考文献

Cyclic-di-GMP 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.4484 mL 7.2421 mL 14.4841 mL 36.2104 mL
5 mM 0.2897 mL 1.4484 mL 2.8968 mL 7.2421 mL
10 mM 0.1448 mL 0.7242 mL 1.4484 mL 3.6210 mL
15 mM 0.0966 mL 0.4828 mL 0.9656 mL 2.4140 mL
20 mM 0.0724 mL 0.3621 mL 0.7242 mL 1.8105 mL
25 mM 0.0579 mL 0.2897 mL 0.5794 mL 1.4484 mL
30 mM 0.0483 mL 0.2414 mL 0.4828 mL 1.2070 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cyclic-di-GMP61093-23-0c-di-GMP cyclic diguanylate 5GP-5GPSTINGEndogenous MetaboliteStimulator of Interferon GenesTMEM173MITAERISMPYSimmunotherapymalignant melanomaH508Jurkat cellscancerCD4Inhibitorinhibitorinhibit

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