2. Cell Cycle/DNA Damage
  3. DNA Stain
  4. DAPI (dihydrochloride) (solution)

DAPI (dihydrochloride) (solution)  (Synonyms: 4',6-Diamidino-2-phenylindole dihydrochloride (solution))

目录号: HY-D0814A
产品使用指南

摩尔计算器

DAPI dihydrochloride (4',6-Diamidino-2-phenylindole dihydrochloride) (solution) 是一种 DAPI 染料。DAPI 是一种能够与 DNA 强力结合的荧光染料。DAPI 结合双链 DNA 小沟的 A/T 碱基对 (1 个 DAPI 分子可以占据 3 个碱基对的位置)，而显使荧光强度放大约 20 倍。通过观测荧光强度，可以对 DNA 定量。DAPI 还可以透过完整的细胞膜，常被用于活细胞和固定细胞的染色。

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DAPI (dihydrochloride) (solution) Chemical Structure

DAPI (dihydrochloride) (solution) Chemical Structure

CAS No. : 28718-90-3

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规格 价格 是否有货
500 μg (28.55 μM * 50 mL in DDH2O) ￥440
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5 mg (28.55 μM * 500 mL in DDH2O) ￥3000
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DAPI (dihydrochloride) (solution) 的其他形式现货产品:

Customer Review

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DAPI (dihydrochloride) (solution) 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 58 篇科研文献

IF

    DAPI (dihydrochloride) (solution) purchased from MCE. Usage Cited in: Pharmaceuticals. 2022 Jun 6;15(6):721.

    The nuclei are counterstained with DAPI (10 μM) in the dark for 10 min at room temperature. Fluorescence is monitored using confocal microscope, and foci of phospho-H2A.X was quantified using ImageJ software.

    DAPI (dihydrochloride) (solution) purchased from MCE. Usage Cited in: Acta Pharmacol Sin. 2021 Jul;42(7):1111-1123.  [Abstract]

    Mice intestine tissues sections were incubated with a TUNEL reaction mixture for 1 h at 37 °C in the dark. Next, the sections are stained with 0.1 μg/mL DAPI.

    DAPI (dihydrochloride) (solution) purchased from MCE. Usage Cited in: Oncotargets Ther. 2020 Oct 6;13:9941-9952.  [Abstract]

    Human GC MGC-803 and NCI-N87 cells are seeded into 24-well plates with glass slide for 24h in RPMI-1640 medium. And 0.1% DAPI staining is performed against light for 30 minutes.

    DAPI (dihydrochloride) (solution) purchased from MCE. Usage Cited in: Acta Biomater. 2019 Sep 15;96:468-479.  [Abstract]

    CLSM observations of tested Cy5-nanocomplexes incubated with RAW 264.7 macrophages show the fluorescent distributions of DAPI, nanocomplexes, and ROS (DCFH-DA) in the presence and absence NIR treatment.

    DAPI (dihydrochloride) (solution) purchased from MCE. Usage Cited in: Phytomedicine. 2020 Mar;68:153146.  [Abstract]

    MC3T3-E1 cells incubated with 1 μg/ml DAPI dihydrochloride for 5 min and observed them under a fluorescence microscope. Effects of sweroside on ER-α and GPR30 expressions by immunofluorescence, white and yellow arrows mean target protein distribution in the cell nuclei and membrane, respectively.

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    DAPI dihydrochloride (4',6-Diamidino-2-phenylindole dihydrochloride) (solution) is a DAPI dye. DAPI is a fluorescent dye that can bind strongly to DNA. DAPI binds to the A/T base pairs in the minor groove of double-stranded DNA (1 DAPI molecule can occupy 3 base pairs), which amplifies the fluorescence intensity by about 20 times. By observing the fluorescence intensity, DNA can be quantified. DAPI can also penetrate intact cell membranes and is often used to stain living and fixed cells[1].

    分子量

    350.25

    Formula

    C16H17Cl2N5
    CAS 号
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    Please store the product under the recommended conditions in the Certificate of Analysis.
    纯度 & 产品资料
    参考文献

    DAPI (dihydrochloride) (solution) 相关分类

    • 摩尔计算器

    • 稀释计算器

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量   浓度   体积   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DAPI (dihydrochloride) (solution)28718-90-34',6-Diamidino-2-phenylindole dihydrochloride (solution)DNA StainInhibitorinhibitorinhibit

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