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  4. DFHO

DFHO 是 Corn 荧光适体的荧光配体。RNA 适体, Corn 结合 DFHO 的 Kd 值为 70 nM，并将其转化为荧光形式，从而使 RNA 在细胞中成像。

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DFHO Chemical Structure

DFHO Chemical Structure

CAS No. : 1420815-34-4

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规格 价格 是否有货 数量
1 mg ￥1150
In-stock
5 mg ￥2500
In-stock
10 mg ￥4000
In-stock
25 mg 现货 询价
50 mg   询价  
100 mg   询价  

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Customer Review

DFHO 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

DFHO is a fluorogenic ligand of Corn fluorogenic aptamer. The RNA aptamer, Corn binds DFHO with a Kd value of 70 nM and converts it to a fluorescent form, enabling RNA imaging in cells[1][2].

IC50 & Target

Kd: 70 nM (Corn binds DFHO)[1]
体外研究
(In Vitro)

Corn is tested in E. coli. Minimal fluorescence that is seen in DFHO-treated cells (20 μM) transformed with the empty vector. However robust yellow fluorescence is seen in E. coli expressing Corn[1].
Corn-DFHO exhibits markedly enhanced photostability in vitro. The Corn-DFHO complexes are imaged in mammalian cells. Since the U6 promoter is one of the three major Pol III promoters, Corn from the U6 promoter is expressed and replaced the majority of the U6 sequence with Corn, leaving a minimal 5′ capping element leader sequence. The majority of the cells expressed diffuse yellow fluorescence, most prominently in the cytoplasm)[1].
DFHO exhibits low background fluorescence and cytotoxicity, making it a potentially suitable fluorophore for activation by RNA aptamers and for imaging experiments in living cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

DFHO 相关抗体:
分子量

281.21

Formula

C12H9F2N3O3
CAS 号
性状

固体

颜色

Yellow to brown

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 100 mg/mL (355.61 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.5561 mL 17.7803 mL 35.5606 mL
5 mM 0.7112 mL 3.5561 mL 7.1121 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

DFHO 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5561 mL 17.7803 mL 35.5606 mL 88.9015 mL
5 mM 0.7112 mL 3.5561 mL 7.1121 mL 17.7803 mL
10 mM 0.3556 mL 1.7780 mL 3.5561 mL 8.8902 mL
15 mM 0.2371 mL 1.1854 mL 2.3707 mL 5.9268 mL
20 mM 0.1778 mL 0.8890 mL 1.7780 mL 4.4451 mL
25 mM 0.1422 mL 0.7112 mL 1.4224 mL 3.5561 mL
30 mM 0.1185 mL 0.5927 mL 1.1854 mL 2.9634 mL
40 mM 0.0889 mL 0.4445 mL 0.8890 mL 2.2225 mL
50 mM 0.0711 mL 0.3556 mL 0.7112 mL 1.7780 mL
60 mM 0.0593 mL 0.2963 mL 0.5927 mL 1.4817 mL
80 mM 0.0445 mL 0.2223 mL 0.4445 mL 1.1113 mL
100 mM 0.0356 mL 0.1778 mL 0.3556 mL 0.8890 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DFHO1420815-34-4Fluorescent DyeCornyellowfluorescenceligandRNAdimerizationimagingliving cellsaptamerRFPInhibitorinhibitorinhibit

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目录号：
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