DOI hydrochloride 

DOI hydrochloride is a serotonin 5-HT_2A/_2C receptor agonist with K_i values of 0.7 nM, 2.4 nM and 20 nM for h5-HT_2A, h5-HT_2C and h5-HT_2B receptors, respectively. DOI hydrochloride can across the blood-brain barrier. DOI hydrochloride increases head twitches in mice^[1][2][3].

DOI hydrochloride (10 μM; for 45 min) causes a massive increase in adhesion with 99% of the cells staying adherent compared to only 42% of untreated Hu2AAB1 cells^[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

DOI hydrochloride (0.3-10 mg/kg; i.p.; single dose) produces an increase in mean head twitches at 3 mg/kg in Wild type (WT) ICR (CD-1) KO mice. Lower doses produces non-significant increases in head twitches, while a higher dose of 10 mg/kg produces increased variability yet a significant increase in mean head twitches^[3].
DOI (0.35-0.7 mmol/kg; sc) significantly increases waking and light sleep and reduces slow wave sleep, rapid-eye-movement (REM) sleep, and the number of REM periods in Male Wistar rats weighing 350-400 g^[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

357.62

C₁₁H₁₇ClINO₂

42203-78-1

固体

White to light yellow

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

• [1]. D L Nelson, et al. Comparisons of hallucinogenic phenylisopropylamine binding affinities at cloned human 5-HT2A, -HT(2B) and 5-HT2C receptors. Naunyn Schmiedebergs Arch Pharmacol. 1999 Jan;359(1):1-6.  [Content Brief]

  [2]. S L Shepheard, et al. Investigation of the effects of IVth ventricular administration of the 5-HT2 agonist, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI), on autonomic outflow in the anaesthetized cat. Br J Pharmacol. 1991 Oct;104(2):367-72.  [Content Brief]

  [3]. Mark J Benvenga, et al. Metabotropic Glutamate 2 Receptors Play a Key Role in Modulating Head Twitches Induced by a Serotonergic Hallucinogen in Mice. Front Pharmacol. 2018 Mar 15;9:208.  [Content Brief]

  [4]. Jaime M Monti, et al. Effects of the serotonin 5-HT2A/2C receptor agonist DOI and of the selective 5-HT2A or 5-HT2C receptor antagonists EMD 281014 and SB-243213, respectively, on sleep and waking in the rat. Eur J Pharmacol. 2006 Dec 28;553(1-3):163-70.  [Content Brief]

  [5]. Joe Anand Kumar John Jayakumar, et al. Serotonin 2A (5-HT 2A) receptor affects cell-matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells. Sci Rep. 2020 Dec 10;10(1):21675.  [Content Brief]