2. Academic Validation
  3. Serotonin 2A (5-HT2A) receptor affects cell-matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells

Serotonin 2A (5-HT2A) receptor affects cell-matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells

  • Sci Rep. 2020 Dec 10;10(1):21675. doi: 10.1038/s41598-020-78595-6.
Joe Anand Kumar John Jayakumar 1 2,  Mitradas M Panicker 3 4,  Basudha Basu 5 6 7
Affiliations

Affiliations

  • 1 Manipal Academy of Higher Education, Manipal, India. joeanandkumarj@gmail.com.
  • 2 National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India. joeanandkumarj@gmail.com.
  • 3 National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India.
  • 4 Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA, USA.
  • 5 Manipal Academy of Higher Education, Manipal, India. b.basu@leeds.ac.uk.
  • 6 National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India. b.basu@leeds.ac.uk.
  • 7 Leeds Institute of Medical Research at St. James's, Faculty of Medicine and Health, Leeds University, Leeds, UK. b.basu@leeds.ac.uk.
PMID: 33303826 DOI: 10.1038/s41598-020-78595-6
Abstract

5-HT2A, a G-protein coupled receptor, is widely expressed in the human body, including in the gastrointestinal tract, platelets and the nervous system. It mediates various functions, for e.g. learning, memory, mood regulation, platelet aggregation and vasoconstriction, but its involvement in cell-adhesion remains largely unknown. Here we report a novel role for 5-HT2A in cell-matrix adhesion.In HEK293 cells, which are loosely adherent, expression and stimulation of human or rat 5-HT2A receptor by agonists such as serotonin or 2,5-dimethoxy-4-iodoamphetamine (DOI) led to a significant increase in adhesion, while inhibition of 5-HT2A by antipsychotics, such as risperidone, olanzapine or chlorpromazine prevented it. 5-HT2A activation gave rise to stress fibers in these cells and was also required for their maintenance. Mechanistically, the 5-HT2A-mediated adhesion was mediated by downstream PKC and Rho signaling. Since 5-HT2A is associated with many disorders such as dementia, depression and schizophrenia, its role in cell-matrix adhesion could have implications for neural circuits.

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