2. Academic Validation
  3. N-methylnicotinamide and nicotinamide N-methyltransferase are associated with microRNA-1291-altered pancreatic carcinoma cell metabolome and suppressed tumorigenesis

N-methylnicotinamide and nicotinamide N-methyltransferase are associated with microRNA-1291-altered pancreatic carcinoma cell metabolome and suppressed tumorigenesis

  • Carcinogenesis. 2014 Oct;35(10):2264-72. doi: 10.1093/carcin/bgu174.
Hui-Chang Bi 1,  Yu-Zhuo Pan 2,  Jing-Xin Qiu 3,  Kristopher W Krausz 4,  Fei Li 4,  Caroline H Johnson 4,  Chang-Tao Jiang 5,  Frank J Gonzalez 4,  Ai-Ming Yu 6
Affiliations

Affiliations

  • 1 School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China, Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
  • 2 Department of Pharmaceutical Sciences, SUNY-Buffalo, Buffalo, NY 14214, USA, Department of Biochemistry & Molecular Medicine, UC Davis Medical Center, Sacramento, CA 95817, USA and.
  • 3 Department of Pathology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
  • 4 Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
  • 5 School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
  • 6 Department of Pharmaceutical Sciences, SUNY-Buffalo, Buffalo, NY 14214, USA, Department of Biochemistry & Molecular Medicine, UC Davis Medical Center, Sacramento, CA 95817, USA and aimyu@ucdavis.edu.
PMID: 25115443 DOI: 10.1093/carcin/bgu174
Abstract

The cell metabolome comprises abundant information that may be predictive of cell functions in response to epigenetic or genetic changes at different stages of cell proliferation and metastasis. An unbiased ultra-performance liquid chromatography-mass spectrometry-based metabolomics study revealed a significantly altered metabolome for human pancreatic carcinoma PANC-1 cells with gain-of-function non-coding microRNA-1291 (miR-1291), which led to a lower migration and invasion capacity as well as suppressed tumorigenesis in a xenograft tumor mouse model. A number of metabolites, including N-methylnicotinamide, involved in nicotinamide metabolism, and l-carnitine, isobutyryl-carnitine and isovaleryl-carnitine, involved in fatty acid metabolism, were elevated in miR-1291-expressing PANC-1. Notably, N-methylnicotinamide was elevated to the greatest extent, and this was associated with a sharp increase in nicotinamide N-methyltransferase (NNMT) mRNA level in miR-1291-expressing PANC-1 cells. In addition, expression of NNMT mRNA was inversely correlated with pancreatic tumor size in the xenograft mouse model. These results indicate that miR-1291-altered PANC-1 cell function is associated with the increase in N-methylnicotinamide level and NNMT expression, and in turn NNMT may be indicative of the extent of pancreatic carcinogenesis.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z