2. Academic Validation
  3. Effect of cell-extracellular matrix interaction on myogenic characteristics and artificial skeletal muscle tissue

Effect of cell-extracellular matrix interaction on myogenic characteristics and artificial skeletal muscle tissue

  • J Biosci Bioeng. 2020 Jul;130(1):98-105. doi: 10.1016/j.jbiosc.2020.02.008.
Ran Ding 1,  Masanobu Horie 2,  Sumire Nagasaka 3,  Saki Ohsumi 3,  Kazunori Shimizu 3,  Hiroyuki Honda 4,  Eiji Nagamori 5,  Hideaki Fujita 6,  Takuo Kawamoto 7
Affiliations

Affiliations

  • 1 Graduate School of Human and Environmental, Kyoto University, Yoshida-Konoe-Cho, Sakyo-ku, Kyoto 606-8507, Japan.
  • 2 Division of Biochemical Engineering, Radioisotope Research Center, Kyoto University, Yoshida-Konoe-Cho, Sakyo-ku, Kyoto 606-8507, Japan. Electronic address: horie.masanobu.4z@kyoto-u.ac.jp.
  • 3 Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan.
  • 4 Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan; Innovative Research Center for Preventive Medical Engineering, Nagoya University, Nagoya 464-8601, Japan.
  • 5 Department of Biomedical Engineering, Osaka Institute of Technology, 5-16-1 Omiya, Asahi-ku, Osaka 535-8585, Japan.
  • 6 WPI, Immunology Frontier Research Center, Institute of Scientific and Industrial Research Center, Osaka University, Suita, Osaka 565-0871, Japan.
  • 7 Graduate School of Human and Environmental, Kyoto University, Yoshida-Konoe-Cho, Sakyo-ku, Kyoto 606-8507, Japan; Division of Biological Chemistry, Radioisotope Research Center, Kyoto University, Yoshida-Konoe-Cho, Sakyo-ku, Kyoto 606-8507, Japan.
PMID: 32278672 DOI: 10.1016/j.jbiosc.2020.02.008
Abstract

Although various types of artificial skeletal muscle tissue have been reported, the contractile forces generated by tissue-engineered artificial skeletal muscles remain to be improved for biological model and clinical applications. In this study, we investigated the effects of extracellular matrix (ECM) and supplementation of a small molecule, which has been reported to enhance α7β1 Integrin expression (SU9516), on cell migration speed, cell fusion rate, myoblast (mouse C2C12 cells) differentiation and contractile force generation of tissue-engineered artificial skeletal muscles. When cells were cultured on varying ECM coated-surfaces, we observed significant enhancement in the migration speed, while the myotube formation (differentiation ratio) decreased in all except for cells cultured on Matrigel coated-surfaces. In contrast, SU9516 supplementation resulted in an increase in both the myotube width and differentiation ratio. Following combined culture with a Matrigel-coated surface and SU9516 supplementation, myotube width was further increased. Additionally, contractile forces produced by the tissue-engineered artificial skeletal muscles was augmented following combined culture. These findings indicate that regulation of the cell-ECM interaction is a promising approach to improve the function of tissue-engineered artificial skeletal muscles.

Keywords

C2C12 cells; Extracellular matrix; SU9516; Skeletal muscle tissue engineering; α7β1 integrin.

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