1. Academic Validation
  2. MicroRNA-315-5p promotes rice black-streaked dwarf virus infection by targeting a melatonin receptor in the small brown planthopper

MicroRNA-315-5p promotes rice black-streaked dwarf virus infection by targeting a melatonin receptor in the small brown planthopper

  • Pest Manag Sci. 2021 Jul;77(7):3561-3570. doi: 10.1002/ps.6410.
Jianhua Zhang  # 1 Yan Dong  # 1 Man Wang 1 Haitao Wang 1 Dianshan Yi 2 Yijun Zhou 1 Qiufang Xu 1
Affiliations

Affiliations

  • 1 Institute of Plant Protection, Key Laboratory of Food Quality and Safety of Jiangsu Province, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu Province, 210014, China.
  • 2 Nanjing Plant Protection and Quarantine Station, Nanjing, Jiangsu Province, 210019, China.
  • # Contributed equally.
Abstract

Background: MicroRNAs (miRNAs), a class of small non-coding endogenous RNAs, play key roles in various biological processes. Most plant viruses are transmitted by insect vectors. However, little is known about the function of miRNAs on plant virus-insect host interaction.

Results: We investigated the role of miR-315-5p in regulation of plant viral Infection in insects using a rice black-streaked dwarf virus (RBSDV) and small brown planthopper (SBPH) interaction system. Our results showed that miR-315-5p had the highest expression level in 2nd-instar nymph, and was highly expressed in the salivary gland and midgut in SBPH. miR-315-5p was in response to and regulated RBSDV Infection in SBPH. Injection of miR-315-5p mimic, agomir-315, significantly increased the RBSDV accumulation, whereas injection of miR-315-5p inhibitor, antagomir-315, reduced virus accumulation in SBPH. Furthermore, a Melatonin Receptor was identified as a target gene of miR-315-5p by the dual luciferase reporter assay. Knockdown of the Melatonin Receptor significantly increased the expression of RBSDV coat protein gene S10 and replication related genes, S5-1, S6, and S9-1. Furthermore, treatment with Melatonin Receptor antagonist luzindole and activator agomelatine significantly increased and reduced RBSDV accumulation in SBPH, respectively. Compared to the control, miR-315-5p did not affect the efficiency of RBSDV acquisition in SBPH. However, the efficiency of RBSDV transmission was significantly reduced after injecting antagomir-315.

Conclusion: Taken together, our data reveal that miR-315-5p is beneficial for RBSDV Infection in its insect vector by directly targeting a Melatonin Receptor. These findings provide a new insight to the function of miRNAs in virus-insect vector interaction. © 2021 Society of Chemical Industry.

Keywords

melatonin receptor; microRNA-315-5p; rice black-streaked dwarf virus; small brown planthopper.

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