2. Academic Validation
  3. Medioresinol as a novel PGC-1α activator prevents pyroptosis of endothelial cells in ischemic stroke through PPARα-GOT1 axis

Medioresinol as a novel PGC-1α activator prevents pyroptosis of endothelial cells in ischemic stroke through PPARα-GOT1 axis

  • Pharmacol Res. 2021 Jul;169:105640. doi: 10.1016/j.phrs.2021.105640.
Yunjie Wang 1,  Xin Guan 2,  Cheng-Long Gao 2,  Wenchen Ruan 2,  Shunyi Zhao 2,  Guoyin Kai 3,  Fei Li 4,  Tao Pang 5
Affiliations

Affiliations

  • 1 State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases, Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, New drug screening center, Jiangsu Center for Pharmacodynamics Research and Evaluation, Institute of Pharmaceutical Sciences, China Pharmaceutical University, Nanjing 210009, PR China; College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 311402, PR China; Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University, Yantai 264005, PR China; School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai 264005, PR China.
  • 2 State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases, Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, New drug screening center, Jiangsu Center for Pharmacodynamics Research and Evaluation, Institute of Pharmaceutical Sciences, China Pharmaceutical University, Nanjing 210009, PR China.
  • 3 College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 311402, PR China.
  • 4 State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases, Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, New drug screening center, Jiangsu Center for Pharmacodynamics Research and Evaluation, Institute of Pharmaceutical Sciences, China Pharmaceutical University, Nanjing 210009, PR China. Electronic address: lifei@cpu.edu.cn.
  • 5 State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases, Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, New drug screening center, Jiangsu Center for Pharmacodynamics Research and Evaluation, Institute of Pharmaceutical Sciences, China Pharmaceutical University, Nanjing 210009, PR China; Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, PR China. Electronic address: tpang@cpu.edu.cn.
PMID: 33915296 DOI: 10.1016/j.phrs.2021.105640
Abstract

Aim: Brain microvascular endothelial cells (BMVECs), as the important structure of blood-brain barrier (BBB), play a vital role in ischemic stroke. Pyroptosis of different cells in the brain may aggravate cerebral ischemic injury, and PGC-1α plays a major role in Pyroptosis. However, it is not known whether BMVECs undergo Pyroptosis after ischemic stroke and whether PGC-1α Activator Medioresinol (MDN) we discovered may be useful against Pyroptosis of endothelial cells and ischemic brain injury.

Methods: For in vitro experiments, the bEnd.3 cells and BMVECs under oxygen and glucose-deprivation (OGD) were treated with or without MDN, and the LDH release, tight junction protein degradation, GSDMD-NT membrane location and pyroptosis-associated proteins were evaluated. For in vivo experiments, mice underwent transient middle cerebral artery occlusion (tMCAO) for ischemia model, and the neuroprotective effects of MDN were measured by infarct volume, the permeability of BBB and Pyroptosis of BMVECs. For mechanistic study, effects of MDN on the accumulation of phenylalanine, mitochondrial Reactive Oxygen Species (mtROS) were tested by untargeted metabolomics and MitoSOX Red probe, respectively.

Results: BMVECs underwent Pyroptosis after ischemia. MDN dose-dependently activated PGC-1α, significantly reduced Pyroptosis, mtROS and the expressions of pyroptosis-associated proteins (NLRP3, ASC, cleaved Caspase-1, IL-1β, GSDMD-NT), and increased ZO-1 and Occludin protein expressions in BMVECs. In tMCAO mice, MDN remarkably reduced brain infarct volume and the permeability of BBB, inhibited Pyroptosis of BMVECs, and promoted long-term neurobehavioral functional recovery. Mechanistically, MDN promoted the interaction of PGC-1α with PPARα to increase PPARα nuclear translocation and transcription activity, further increased the expression of GOT1 and PAH, resulting in enhanced phenylalanine metabolism to reduce the ischemia-caused phenylalanine accumulation and mtROS and further ameliorate Pyroptosis of BMVECs.

Conclusion: In this study, we for the first time discovered that Pyroptosis of BMVECs was involved in the pathogenesis of ischemic stroke and MDN as a novel PGC-1α Activator could ameliorate the Pyroptosis of endothelial cells and ischemic brain injury, which might attribute to reduction of mtROS through PPARα/GOT1 axis in BMVECs. Taken together, targeting endothelial Pyroptosis by MDN may provide alternative therapeutics for brain ischemic stroke.

Keywords

Endothelial cells; GSK0660 (PubChem CID: 46233311); GW6471 (PubChem CID: 446738); GW9662 (PubChem CID: 644213); Ischemic stroke; Medioresinol; Medioresinol (PubChem CID: 181681); PGC-1α; PPARα; Phenylalanine; Phenylalanine (PubChem CID: 6140). More information is available at: https://www.elsevier.com/PubChem; Pyroptosis; ZLN005 (PubChem CID: 899323).

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-15372
    99.64%, PPAR拮抗剂
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z