1. Academic Validation
  2. Curcumin inhibits classical swine fever virus replication by interfering with lipid metabolism

Curcumin inhibits classical swine fever virus replication by interfering with lipid metabolism

  • Vet Microbiol. 2021 Aug;259:109152. doi: 10.1016/j.vetmic.2021.109152.
Ya Gao 1 Jia-Huan Hu 1 Xiao-Dong Liang 1 Jing Chen 1 Chun-Chun Liu 1 Ya-Yun Liu 1 Yan Cheng 1 Yun Young Go 2 Bin Zhou 3
Affiliations

Affiliations

  • 1 MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • 2 Department of Infectious Diseases and Public Health, City University of Hong Kong, Hong Kong, China.
  • 3 MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China. Electronic address: zhoubin@njau.edu.cn.
Abstract

Although previous reports have shown that Curcumin inhibits many viruses, including some important members of different genera of Flaviviridae family (Japanese encephalitis virus, Dengue virus and hepatitis C virus), the Antiviral activity of curcumin against Classical swine fever virus (CSFV), which belongs to Pestivirus genus, is still unclear. In this study, we found that curcumin inhibited CSFV replication in a dose-dependent manner, but had no effect on virus adsorption and entry. Furthermore, the results showed that curcumin inhibited the expression of FASN, one of the key Enzymes of fatty acid synthesis pathway, thereby, causing the reduction of the production of LDs upon Infection. To this end, we detected transcription factor 6 (ATF6), the key factor of regulating lipid metabolism along with other related molecules (CHOP and GPR78) and found that curcumin significantly impaired the gene synthesis of ATF6, while CSFV Infection promoted ATF6 expression. Therefore, it is confirmed that curcumin inhibited CSFV replication by interfere lipid metabolism. In addition, our subsequent studies found that curcumin played an Antiviral role by promoting the innate immune independent of NF-κB signaling pathway. Taken together, our finding highlights that curcumin is a potential candidate drug against CSFV for controlling CSF.

Keywords

Antiviral activity; Classical swine fever virus (CSFV); Curcumin; Innate immunity; Lipid metabolism.

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