2. Academic Validation
  3. The immunomodulatory activity of degradation products of Sesbania cannabina galactomannan with different molecular weights

The immunomodulatory activity of degradation products of Sesbania cannabina galactomannan with different molecular weights

  • Int J Biol Macromol. 2022 Apr 30;205:530-538. doi: 10.1016/j.ijbiomac.2022.02.122.
Yuheng Tao 1,  Junmei Ma 2,  Caoxing Huang 1,  Chenhuan Lai 1,  Zhe Ling 2,  Qiang Yong 3
Affiliations

Affiliations

  • 1 Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Key Laboratory of Forestry Genetics & Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
  • 2 Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
  • 3 Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Key Laboratory of Forestry Genetics & Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing 210037, People's Republic of China. Electronic address: swhx@njfu.com.cn.
PMID: 35217078 DOI: 10.1016/j.ijbiomac.2022.02.122
Abstract

Galactomannan (GM) is widely recognized as an immune enhancer; however, the underlying molecular mechanism is still unknown. Herein, four products with molecular weights in descending order, namely GM40, GM50, GM65, and GMOS, were separated from incomplete degradation products of Sesbania cannabina GM by ethanol precipitation, followed by their immunomodulatory activity. Through FTIR and XPS spectra, the amount of free hydroxyl groups was shown to decrease in the following order: GM > GM50 > GMOS > GM40 > GM65. Moreover, the immunomodulatory activity of different products decreased in abovementioned order. The TNF-α, IL-6 and TLR4 content in RAW 264.7 cells treated with different GM products in the presence or absence of TAK-242 (TLR4 Inhibitor) suggested that the immunomodulatory activity of GM and its degradation products is TLR4-dependent. Overall, the preliminary relationship indicated here between the hydroxyl groups or the possible deeper structural changes of GM and the immunomodulatory activity need to be further investigated.

Keywords

Galactomannan; Hydroxyl group; Toll-like receptor 4.

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